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The production of point-of-care nucleic acid tests based on the detection of miRNAs is therefore heavily dependent on the development of a chip-based sample processing device that would allow extraction or pre-purification of the biological samples prior to reaching the sensing platform. In this report, we classify and analyze the most promising technologies that have been developed to help facilitate the migration of nucleic acid tests based on miRNA detection from bench to bedside.
Source link: https://europepmc.org/article/MED/35048934
Nucleic acid extraction at point-of-care is highly recommended for implementing nucleic acid extraction at point of care by cheap, reliable, easy, portable, and equipment-free. In addition, researching the correlation between the physical and chemical properties of paper-based materials and the NAE efficiency of paper-based papers is instructive in the production of new paper-based NAE materials. We first systematically compared six commonly used paper-based NAE products, then rated their NAE performance, then evaluated their NAE effectiveness. With high concentration and purity, the NAE's performance of FTA card is the best ever, with high concentration and purity.
Source link: https://europepmc.org/article/MED/35125684
RNA sequencing allows for the quantification of embryo transcriptomes in order to investigate transcriptional responses to various perturbations. We've devised a method to extract complete nucleic acid from individual zebrafish embryos. The total nucleic acid can be genotyped and then DNase I treated to obtain RNA samples for sequencing. Graphic summary: Total nucleic acid extraction from individual zebrafish embryos for genotyping and RNA-seq.
Source link: https://europepmc.org/article/MED/35118175
In recent years, the use of nanopore technologies for the detection of organic biogenic compounds has drew significant attention. In this research, we investigated pre-existing, automated, or semiautomated NA extraction methods, as well as automated ONT VolTRAX NA sample preparation and validation with Nanopore MinION sequencing. DNA from Canadian High Arctic environments ähning to Mars, Europa, and Enceladus can be extracted and sequenced with the MinION, according to the testing results. All of the extraction techniques tested showed promise for extracting DNA from Canadian High Arctic environments analogous to Mars, Europa, and Enceladus, which could be later found and sequenced with the MinION. Overall, bead beating-based methods did the best for these parameters. In addition, we found that the MinION could sequence unpurified DNA embedded in crude cell lysates. Our results show that semiautomated NA extraction and preparation methods have a lot of promise, and that with increased automation and automation, can be correlated to a larger platform that incorporates nanopore detection and sequencing of NAs for life detection applications.
Source link: https://europepmc.org/article/MED/34962136
Using Flinders Technology Associates cards for nucleic acid extraction and recombinase, a slidable centrifugal disc was developed for automated and multi-channel detection of Salmonella typhimurium in this research. The slidable FTA card conversion and centrifugal fluidic control were elaborately combined to produce fully automatic operations, including centrifugation of the bacterial sample to extract the nucleic acids, heating and drying of the FTA card to remove the impurities, washing of the FTA card to remove the impurities, and RAA analysis of the extracted DNA to determine the concentration.
Source link: https://europepmc.org/article/MED/34796896
paraphrasedoutput:BACKGROUND: The development of an automated nucleic acid extraction process has numerous benefits over traditional manual methods. This research was conducted to determine the effectiveness of two different techniques for nucleic acid extraction in the virus transport medium. METHODS: For the detection of SARS-CoV-2, we obtained 20 nasopharyngeal swabs in a viral transport medium from the Asia University Hospital's emergency department for the detection of SARS-CoV-2. The results of the MaelstromTM 8 and the QIAamp Viral RNA Mini Kit were compared to the extraction of nucleic acid from viral transport medium. The mean cycle threshold value of the two techniques was 29. 1. CONCLUSIONS: The MaelstromTM 8 and the QIAamp Viral RNA Mini Kit's operation values were similar to each other, and the QIAamp Viral RNA Mini Kit's performance values were similar to each other. The MaelstromTM 8 is more suitable for clinical laboratories that use small or medium-sized samples for nucleic acid extraction.
Source link: https://europepmc.org/article/MED/34910440
Over 240 million COVID-19 cases were recorded as of October 2021, with 4. 9 mln deaths. COVID-19, a novel coronavirus named SARS-CoV-2, is a novel coronavirus that has been described as a novel coronavirus. This research tested two RNA extraction techniques and four commercial RT-qPCR assays commonly used in diagnostic laboratories for detecting SARS-CoV-2 in human specimens from the upper respiratory tract. Our research showed the positive results of the laboratory's diagnostic procedures on the SARS-CoV-2 detection in clinical specimens with low viral loads.
Source link: https://europepmc.org/article/MED/34943484
Fecal DNA testing stands out among many screening methods due to its high sensitivity. The comparative study using the centrifugal adsorption column method revealed the inherent advantages of the magnetic bead method in extracting fecal DNA. The DNA obtained by the magnetic bead method is of high quality, can also be used for high-throughput measurements, and is more suitable for polymerase chain reaction detection, greatly reducing the stool DNA testing process and providing the basis for widespread early screening.
Source link: https://europepmc.org/article/MED/34900193
A rapid DNA extraction technique, recombinase aided in amplification, and a chemical heating packet were integrated and identified as RRC platform for on-site detection of nucleic acid. SHZD32-1 was discovered on the novel platform for demonstration purposes, demonstrating its reliability and capability for on-site detection. High-quality DNA suitable for molecular analysis was quickly extracted in 3-5 minutes using the RDEM. The heat generated by CHP exceeded RAA's temperature requirements.
Source link: https://europepmc.org/article/MED/34881235
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