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Acetylation - DOAJ

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Last Updated: 10 November 2022

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Redox state and the sirtuin deacetylases are major factors that regulate the acetylation status of the stress protein NQO1

NQO1's stress induced protein NQO1 can play a variety of biological pathways that are dependent on the interaction of NQO1 with protein targets. When NQO1 was in an oxidized form, it was immediately acetylation by NADH prior to acetylation, but it was shielded from acetylation when NQO1 was in the reduced form. Both sirtuin isoforms and quantitative MS analysis done with SIRT2 showed significant deacetylation of NQO1, specifically at K262 and K271 in the C-terminal zone.

Source link: https://doi.org/10.3389/fphar.2022.1015642


Proteome-wide analyses reveal diverse functions of protein acetylation and succinylation modifications in fast growing stolons of bermudagrass (Cynodon dactylon L.)

BACKGROUND BACTON Bermudagrass is a common warm-season turfgrass species with well-developed stolons, which lay the groundwork for a rapid propagation of bermudagrass plants by asexual clonal expansion. However, the growth and development of bermudagrass stolons however, is still unclear at the molecular level. A total of 4657 lysine acetylation sites on 1914 proteins and 226 lysine succinylation sites on 128 proteins were successfully identified by liquid chromatography and tandem mass spectrometry, respectively. These results partially explained the varying growth disturbances of bermudagrass stolons during treatment with sodium butyrate and sodium malonate, both of which interfere with protein acetylation and succinylation, respectively. The results not only provide new insights into clonal growth of bermudagrass, but also provide a rich resource for functional studies of protein lysine acetylation and succinylation in plants.

Source link: https://doi.org/10.1186/s12870-022-03885-2


Dynamic regulation of KIF15 phosphorylation and acetylation promotes focal adhesions disassembly in pancreatic cancer

Focal adhesion turnover is crucial for cancer cell metastasis, and the integrin recycling process is a key activation pathway for focal adhesion depolymerization. We screened kinesin proteins involved in integrinu3b21 recycling using a kinesin family siRNA library to identify the key regulator and identified kinesin family 15 as a key regulator. Metastasis PC tissues were upregulated in metastasis PC tissues, and PC cell migration and invasion was promoted. We identified KIF15 as a key component mediating integrin, indicating that FA disassembly in a FAK-Y397-dependent manner. These results show that KIF15 works with PI3K-C2u03b1 to promote FA turnover in PC cells by regulating endosome recycling of integrin u03b21 in a SIRT1-dependent manner, eventually promoting focal adhesion turnover and distant metastasis in PCs.

Source link: https://doi.org/10.1038/s41419-022-05338-y


Acetyl coenzyme A kinetic studies on N-acetylation of environmental carcinogens by human N-acetyltransferase 1 and its NAT1*14B variant

Acetyl coenzyme A is a cofactor in the N-acetylation of many carcinogens, such as aromatic amines and alkylanilines, by a xenobiotic metabolizing enzyme. Molecular structure of AcCoA measured towards p-aminobenzoic acid, 4-aminobiphenyl, benzidine, and 3,4-dimethylaniline in the latest study.

Source link: https://doi.org/10.3389/fphar.2022.931323


Effects of Hst3p inhibition in Candida albicans: a genome-wide H3K56 acetylation analysis

With a mortality rate of nearly 40% in Intensive Care Units, it is the third most common worldwide cause of disease in Intensive Care Units. The quest for a more effective antifungal therapy has been stymied by the difficulty of finding molecules that recognize the microbial cell without damaging the host. In C. albicans, Hst3p, a sirtuin that deacetylates H3K56ac, is a common antifungal target since it is necessary for the fungus viability and virulence. H3K56ac gene profiling in C. albicans has resulted in H3K56ac-enriched regions associated with Candida sp. Here, we present the first genome-wide profiling of H3K56ac in C. albicans. In addition, our RNA-seq analysis revealed 1330 upregulated and 1081 downregulated transcripts on Hst3p inhibition, and among them, we discovered 87 genes whose transcriptional increase was closely associated with H3K56 acetylation on their promoters, as well as some well-known regulators of phenotypic switching and virulence.

Source link: https://doi.org/10.3389/fcimb.2022.1031814


Lysine Acetylome Profiling Reveals Diverse Functions of Acetylation in Deinococcus radiodurans

ABSTRACT Lysine acetylation is a highly conserved posttranslational modification that plays essential roles in a variety of biological processes in a variety of organisms. Deinococcus radiodurans, which is also known for its extreme resistance to radiation. However, in D. radiodurans, only one research has focused on lysine acetylation. For a global analysis of lysine acetylation of D. radiodurans, antibody enrichment techniques and high-resolution liquid chromatography mass spectrometry were used in the present study. We produce the biggest acetylome database set in Washington, D. radiodurans to date, with 4,364 acetylated sites containing 1,410 acetylated proteins, containing a total of 4,364 acetylated proteins. Strikingly, 56. 7 percent of proteins isolated by the uniport database, are acetylated in D. radiodurans, according to the author. For the first time, we publish the most comprehensively sine acetylation in D. radiodurans, which is of vital importance to reveal its robust resistance to radiation. ImportANCE D. radiodurans is distinguished as the most radioresistant organism known to date by the most radioresistant organism identified to date. For the first time, we integrated acetyl-lysine enrichment scheme, high-resolution mass spectrometry, and bioinformatics to identify the lysine acetylated proteins. About half of the total annotated proteins have been found in acetylated forms, which is the first acetylome data set reported in D. radiodurans to date. The findings of this research confirm that acetylation plays key regulatory roles in various aspects of the cellular process, particularly DNA damage repair and metabolism. It provides insight into the role of lysine acetylation in the robust resistance to radiation.

Source link: https://doi.org/10.1128/spectrum.01016-21


Acetylation of Lactate Dehydrogenase Negatively Regulates the Acidogenicity of Streptococcus mutans

lysine acetylation can be present in several essential pathways, and it is also important for bacterial virulence. However, the cellular response of lysine acetylation events to bacterial virulence factors remains unclear. We investigated LDH acetylation's regulatory mechanism and characterized the effect of LDH acetylation on its function and characterized the effect of LDH acetylation on its function. The 15 Gcn5 N-acetyltransferases family members in S. mutans were shocked to learn that the acetyltransferase ActA contributed to its acidogenicity by acetylating LDH, according to We overexpressed them. In addition, enzymatic acetyltransferase reactions showed that purified ActA could acetylate LDH in vitro, and ten potential lysine acetylation sites of LDH were identified by mass spectrometry, 70% of which were also found in vivo. We later found that LDH's acetylation inhibited its enzyme synthesis, and a subsequent rat caries model revealed that ActA affected the niogenicity of S. mutans. We found that ActA, the first identified and characterized acetyltransferase in S. mutans, acetylated the LDH enzymatically and inhibited its enzymatic activity, thereby setting the tone for further investigation into the biological significance of lysine acetylation in S. mutans' virulence. We reviewed the acetylome profiles of Streptococcus mutans and found that lactate dehydrogenase contains several lysine acetylation sites. Consequently, LDH acetylation may be a potential target for future novel therapeutics to reduce dental caries.

Source link: https://doi.org/10.1128/mbio.02013-22


H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin

Male germ cells undergo a dramatic chromatin transformation after the nucleo-histone to nucleo-protamine transition, which is required for proper sperm quality. Butyrylation on the same residue has been implicated in temporal control of NH-NP transition in mice, delaying the bromodomain test is specific protein-dependent nucleosome disassembly and possibly distinguishing retained nucleosomes. H4K5bu and H4K5ac in human normal spermatogenesis are discussed here, indicating a specific role for H4K5bu during spermatogenesis, coexisting with H4K5ac but with different starting locations.

Source link: https://doi.org/10.3390/ijms232012398


Effect of Acetylation on the Physical and Mechanical Performances of Mechanical Densified Spruce Wood

The water absorption rate of wood has been reduced by 37% after acetylation, and densification will help decrease the water absorption. The hardness of the densified wood is noticeably higher than that of control wood and rises with increasing compression ratio. Acetylated wood's cell wall is thicker than that of control wood, which could lead to an increase in the wood's compression density. As a result, the hardness and MOR of acetylated densified wood are noticeably higher than those of unacetylated densified wood.

Source link: https://doi.org/10.3390/f13101620


Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes

CC chemokine ligand -1 is one of T helper 2-related chemokines that are involved in the recruitment of Th2 inflammatory cells. Our research sought to investigate the anti-inflammatory effects of 4-PSB-2 on CCL-1 production in human monocytes and identify potential intracellular pathways, as well as epigenetic regulation. To test our hypothesis, human monocyte THP-1 cell line and primary CD14+ cells were pretreated with different concentrations of 4-PSB-2 and then stimulated with lipopolysaccharide. 4-PSB-2 had a suppressive effect on LPS-induced CCL-1 production, according to this research. In addition, 4-PSB-2 could reduce CCL-1 production by epigenetic control by downregulating histone H3 and H4 acetylation. In short, our analysis revealed that 4-PSB-2 may have a potential role in allergic inflammation treatment.

Source link: https://doi.org/10.3390/cimb44100315

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions