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Acetyl Citrate Lyase - Crossref

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Last Updated: 13 November 2022

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Impact of ATP-citrate lyase catalytic activity and serine 455 phosphorylation on histone acetylation and inflammatory responses in human monocytic THP-1 cells

Nonetheless, the effect of phosphorylation on ACLY function in human myeloid cells is uncertain. We reconstituted ACLY knockout human monocytic THP-1 cells with a wild variety ACLY, catalytically inactive H760A, and phosphate-deficient S455A mutants in this research. We determined the effect of ACLY activity and phosphorylation on histone acetylation and pro-inflammatory gene expression in reaction to lipopolysaccharide using cell lines. Our findings indicate that ACLY serine 455 phosphorylation does not have a role in ACLY's proper enzymatic function, since both, the wild type ACLY and phosphatrylation-deficient mutant, showed increased cell growth and acetylation as compared to cells without a loss of ACLY activity. S455A ACLY-expressing cells demonstrated a phenotype very similar to wild type cells at the same time. Contrary to ACLY knockout, pharmacological inhibition of ACLY in THP-1 cells or primary human macrophages does not increase LPS-triggered pro-inflammatory gene expression.

Source link: https://doi.org/10.3389/fimmu.2022.906127


Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp.

Although metabolic engineering was previously shown to be a cost-effective way to increase lipid production in Schizochytrium spp, metabolic engineering has never been used to raise DHA levels. The reporter system with the promoter's involvement characterized four constitutive promoters and one methanol-induced AOX1 promoter as distinct by the reporter system. p> TEF = tEF - 1 p> ubiquitin p. ccg1 p > ubiquitin p. In Schizochytrium sp, the strong constitutive promoter cg1 p, Schizochytrium ATP-citrate lyase and acetyl-CoA carboxylase were overexpressed. DHA yields of the wild-type strain and the corresponding overexpression strains were up by 23. 3, 5. 3, 6. 1, and 6. 4 g/L, respectively, as measured by 23. 3, 41. 9, and 48. 8%. The results show that overexpression of ACL and ACC enhances lipid transport and DHA production in Schizochytrium sp.

Source link: https://doi.org/10.1186/s13068-020-01767-z

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions