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A heterologous pathway for sucrose transport and metabolic process was introduced into \ n Clostridium beijerinckii \ n to enhance sucrose usage for \ n \ n -butanol production. With sucrose, sugarcane molasses and sugarcane juice as substrates, the \ n C. beijerinckii \ n pressure harbouring \ n StSUT1 \ n and \ n SUC2 \ n boosted acetone-- butanol-- ethanol production by 38. 7%, 22. 3% and 52. 8%, specifically, contrasted with the wild-type pressure. This is the first record to demonstrate enhanced sucrose fermentation as a result of the heterologous expression of a sucrose transporter and invertase in \ n Clostridium \ n.
Source link: https://doi.org/10.1098/rsos.201858
In the existing study, we explored the fermentability of milk dirt powder with high lactose content, for ABE production by Clostridium acetobutylicum and Clostridium beijerinckii. Contrasted to fermentation with glucose, fermentation of milk dirt powder enhanced butanol to acetone proportion by 16% and 36% for C. acetobutylicum and C. beijerinckii, respectively. While these outcomes show the fermentability of milk dirt powder, the physico-chemical properties of milk dirt powder showed up to restrict sugar usage, growth and ABE production.
Source link: https://doi.org/10.1186/2193-1801-3-387
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