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ATP Synthase - PubMed

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Last Updated: 10 August 2022

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Chemical Conformation of the Essential Glutamate Site of the c-Ring within Thermophilic Bacillus FoF1-ATP Synthase Determined by Solid-State NMR Based on its Isolated c-Ring Structure.

The rotation of the Fo c-subunit ring, which contains protons in essential acidic amino acid residues, aids in prototyping by the membrane-embedded Fo component of F-type ATP synthase. By solid-state NMR, we established the structure of the isolated thermophilic Bacillus Fo c-ring, consisting of ten subunits, to clarify it based on the chemical conformation of the essential acidic residues of FoF1. Six pairs of cN23 and tFoF1, surrounded by membrane lipids, take the closed form, while the other four in the a-c interface use the deprotonated open form in a proportion of 87%. The four cE56 residues in the a-c interface were estimated as a pKa value of the four cE56 residues in the a-c interface by cN23 signal sensitivity in the open and closed forms as well as the distribution of polar residues around each cE56.

Source link: https://doi.org/10.1021/jacs.2c03580


Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit.

The proton motive force generated by solar energy-driven thylakoid electron transport reactions by Chloroplast ATP synthase converts ATP. We'll look at how increasing ATP synthase affects leaf photosynthesis and rice production, oryza sativa variety Kitaake. When a stepwise increase in CO2 partial pressure was imposed on leaves at high irradiance, plants with increased AtpD content had higher CO2 assimilation rates. Despite a wild type-like abundance of the Cytochrome b6f complex, fitting of the CO2 response curves of assimilation showed that plants overexpressing AtpD had a higher electron transport rate at high CO2 and increased electron transport rate at high CO2. Both increased maximum carboxylation rate and lower cyclic electron flow were found in transgenic plants, contributing to increased ATP production in comparison to wild type plants. Our findings reveal that ATP synthase's activity modulates electron transport at elevated CO2 and high irradiance.

Source link: https://doi.org/10.1093/jxb/erac320


TNK2/ACK1-mediated phosphorylation of ATP5F1A (ATP synthase F1 subunit alpha) selectively augments survival of prostate cancer while engendering mitochondrial vulnerability.

TNK2/ACK1 phosphorylated ATP5F1A at Tyr243 and Tyr246 not only improved the stability of complex V but also increased mitochondrial energy production in cancer cells, but also increased mitochondrial energy production in cancer cells. -9b reversed this process and increased mitophagy-based autophagy to reduce prostate tumor formation while sparing normal prostate cells. As prostate cancer progressed to the malignant stage, a dramatic rise in p-Y-ATP5F1A was seen, consistent with these results.

Source link: https://doi.org/10.1080/15548627.2022.2103961


DJ-1 interacts with the ectopic ATP-synthase in endothelial cells during acute ischemia and reperfusion.

ATP-Synthase is now ectopically expressed in many cell surfaces, with putative roles in angiogenesis, proliferation, and intracellular pH regulation being cited. During acute ischemia and I/R in ECs, we focused on the description of DJ-1's endothelial dynamics and its role in the regulation of the ectopic ATP-synthase gene expression. DJ-1 was discovered only in the mysterious genome of ischemic cells, which was a cleaved version of DJ-1. The inhibition of DJ-1 expression slowed the ecATP-S response to ischemia by 51%, and the exogenous administration maximized the effect, as well as an improved Akt phosphorylation and angiotube-formation potential at reperfusion. Direct contact was observed between DJ-1 and the ecATP-S in the ecATP-S study. According to Altogether, DJ-1 is actively cleared and released from ischemic ECs, and he plays a vital role in the control of the ect-S gene expression during acute ischemia and reperfusion.

Source link: https://doi.org/10.1038/s41598-022-16998-3


Rotational Mechanism of FO Motor in the F-Type ATP Synthase Driven by the Proton Motive Force.

The FOF1 ATP synthase converts the central rotor and promotes conformational changes in the F1 motor, resulting in ATP synthase. Despite high resolution, however, static data alone is not able to clarify how and where the protons pass through the FO and how proton passage is linked to FO rotation. A free energy surface was revealed based on the protonation state and rotational angle of the rotor's coarse-grained MD simulations.

Source link: https://doi.org/10.3389/fmicb.2022.872565


Chronic inhibition of the mitochondrial ATP synthase in skeletal muscle triggers sarcoplasmic reticulum distress and tubular aggregates.

Tubular Aggregate Myopathy patients' skeletal muscle, as well as other conditions including endocrine syndromes, diabetes, and age, being the primary cause unknown. We show that TA is the product of persistent mitochondrial ATP synthase deficiency in muscle muscle. According to studies, hypoxic muscle cultured ex vivo demonstrate an increase in mitochondria/SR contact sites and autophagic/mitophagic zones, where TA clusters form near defective mitochondria. TAM patients' muscle proteome analyses reveal similar shifts in mitochondrial dynamics and in ATP synthase content, which are troubling. Mice with restrained OXPHOS recovered a healthy phenotype by stimulating mitogenesis and mitochondrial fusion. Our results reveal a functional link between the ATP synthase/DRP1 axis and the setting of TA, as well as repurposed edaravone as a potential treatment for TA-related disorders.

Source link: https://doi.org/10.1038/s41419-022-05016-z


ATP synthase subunit e is a shrimp growth-associated breeding marker.

Penaeus vannamei is one of the world's most common aquaculture species. In a hybrid lineage, the relationship between these genes and shrimp growing was further established after that. The expression of two genes, including ATP synthase subunit e and inhibitor of apoptosis protein, showed some association with shrimp growth rate. In addition, we tested these two candidate markers in a variety of lineages and discovered that ATP synthase subunit e could be a shrimp growth-associated breeding marker.

Source link: https://doi.org/10.1016/j.ygeno.2022.110410


Crizotinib Shows Antibacterial Activity against Gram-Positive Bacteria by Reducing ATP Production and Targeting the CTP Synthase PyrG.

Here, we reviewed an FDA-approved small-molecule library and discovered that crizotinib has a strong antimicrobial activity against Gram-positive bacteria. In an animal model, Crizotinib was shown to raise the survival rate of mice infected with bacteria and reduce pulmonary inflammation activity. According to a drug affinity sensitive target stability report, crizotinib targets the CTP synthase PyrG, which then disrupts pyrimidine metabolism and eventually reduces DNA production. The initiation of this CTP synthase occurs in close proximity to PyrG's ATP binding pocket, causing loss of function of the CTP synthase, according to a subsequent molecular dynamics report. Crizotinib is a promising antimicrobial agent and provides a novel alternative for the production of Gram-positive infections. IMPORTANCE Infections caused by drug-resistant bacteria are a significant issue around the world. Crizotinib was discovered to disrupt pyrimidine metabolism by attacking the CTP synthase PyrG, thus reducing DNA synthesis, thus decreasing DNA synthesis.

Source link: https://doi.org/10.1128/spectrum.00884-22


Proteomic analysis of the ATP synthase interactome in notothenioids highlights a pathway that inhibits ceruloplasmin production.

The icefish Champsocephalus gunnari has a significantly higher ATP synthase subunit u03b1 expression than the red-blooded Notothenia rossii, but a much smaller interactome than the other species, according to this report. We investigate the interactionsome of ATP synthase subunit u03b1 in two red-blooded species Trematomus bernacchii, N. rossii, and the icefish Chionodraco rastrospinosus and C. gunnari, finding that compared to other species, there are no oxidative-stress-associated proteins present in the identified interactome of C. gunnari. Western blot is detected in T. bernacchii's liver, but not in N. rossii, C. rastrospinosus, and C. gunnari.

Source link: https://doi.org/10.1152/ajpregu.00069.2022

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions