Advanced searches left 3/3

ATP Synthase - Europe PMC

Summarized by Plex Scholar
Last Updated: 10 August 2022

* If you want to update the article please login/register

Chemical Conformation of the Essential Glutamate Site of the c -Ring within Thermophilic Bacillus F o F 1 -ATP Synthase Determined by Solid-State NMR Based on its Isolated c -Ring Structure.

F o F 1's Cryoelectron microscopy structures reveal a unique proton translocation system. To establish the chemical conformation of these residues in tF o F 1 -ATP synthase, we incorporated stereo-array-isotope-labeled Glu and Asn into the tF o c ring. Six pairs of c N23 and c E56 surrounded by membrane lipids take the closed form, while the other four in the a - c interface use the deprotonated open form in a proportion of 87%, based on the signal strength and configuration of the tF o ring and tF o F 1 o F 1. The p K a values of the four c E56 residues in the a c interface were estimated from the c N23 signal strength in the open and closed forms and distribution of polar residues around each c E56.

Source link: https://europepmc.org/article/MED/35905443


Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit.

The proton motive force generated by solar energy-driven thylakoid electron transport reactions is used to generate ATP by Chloroplast ATP synthase. Here we investigate how increasing ATP synthase influences leaf photosynthesis and growth of rice, Oryza sativa variety Kitaake. When a stepwise increase in CO2 partial pressure was placed on leaves with elevated irradiance, plants with elevated AtpD content had higher CO2 assimilation rates. Despite having a wild type-like abundance of the Cytochrome b6f complex, fitting of the CO2 response curves of assimilation revealed that plants overexpressing AtpD had a higher electron transport rate at high CO2 in high CO2. Both increased maximum carboxylation rate and lower cyclic electron flow in transgenic plants correlated to an elevated ATP production increase compared to wild type plants. Our findings show that ATP synthase activity modulates electron transport rates at elevated CO2 and high irradiance.

Source link: https://europepmc.org/article/MED/35904136


Mutational analysis of a conserved positive charge in the c -ring of E. coli ATP synthase

ABSTRACT F1 F o ATP synthetes adenosine triphosphate, life's basic energy currency, synthetizing it. The electrochemical gradient of protons is turned into rotation by the membrane-embedded F o motor, which is then used to trigger conformational shifts in the soluble F 1 motor that catalyzes ATP synthesis. The F o motor is constructed of a c 10 ring and subordinate a, which combine to form two aqueous half channels that promote proton translocation in E. coli, which are both connected. The proton translocation process is thought to have involved Arg50 and Thr51 on the cytoplasmic side of each subunit c, and positive charge has been conserved in this region of subunit c.

Source link: https://europepmc.org/article/PPR/PPR524730


TNK2/ACK1-mediated phosphorylation of ATP5F1A (ATP synthase F1 subunit alpha) selectively augments survival of prostate cancer while engendering mitochondrial vulnerability.

TNK2/ACK1 phosphorylated ATP5F1A at Tyr243 and Tyr246 not only improved the stability of complex V, but also increased mitochondrial energy production in cancer cells, according to here. phosphat-5F1A blocked its binding to its physiological inhibitor, ATP5IF1, in addition, allowing continued mitochondrial function to promote cancer cell growth. 9b reversed this process and stimulated mitophagy-based autophagy to reduce prostate tumor formation while sparing normal prostate cells, but not in normal prostate cells. Moreover, Tnk2 transgenic mice showed elevated p-Y-ATP5F1A and loss of mitophagy, as well as the development of prostatic intraeoplasia. The prostate cancer epidemic was apparent at the malignant stage, with a notable rise in p-Y-ATP5F1A. Overall, this research revealed the molecular intricacy of tyrosine kinase-mediated mitochondrial energy regulation as a key cancer cell mitochondrial vulnerability and revealed evidence that TNK2 inhibitors can act as "mitocans" to promote cancer-specific mitophagy.

Source link: https://europepmc.org/article/MED/35895804


DJ-1 interacts with the ectopic ATP-synthase in endothelial cells during acute ischemia and reperfusion.

Endothelial cells play a vital role in ischemia. During acute ischemia and I/R in ECs, we focused on the characterization of DJ-1's endothelial process and its role in the regulation of the ectopic ATP-synthase enzyme production. DJ-1 was discovered only in a specificome of ischemic cells, according to a cleaved version of the DJ-1. Following acute ischemia in ECs, the ecATP-S activity soared, coinciding with DJ-1 and DJ-120C secrecy. The inhibition of DJ-1 expression reduced the ecATP-S response to ischemia by 50%, and its exogenous administration maximized the result, as well as an improved Akt phosphorylation and angiotube-formation potential at reperfusion. Direct comparisons were found between DJ-1 and the ecATP-S in the ecclimatization studies. During acute ischemia and reperfusion, Altogether's report reveals that DJ-1 is quickly cleaved and released from ischemic ECs, and plays a key role in the regulation of ecATP-S activity during acute ischemia and reperfusion.

Source link: https://europepmc.org/article/MED/35882968


Beneficial effect of polyphenols in COVID-19 and the ectopic F 1 F O -ATP synthase: Is there a link?

Polyphenols bind and inhibit the F 1 F o-ATP synthase rotary catalysis of polyphenols. The ectopic F 1 F o-ATP synthase expressed on the endothelial plasma membrane, perhaps an early target of polyphenols.

Source link: https://europepmc.org/article/MED/35838055

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions