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Appropriate glucose-stimulated insulin secretion by pancreatic u03b2-cells is a vital component of blood glucose homeostasis. Compared to 2D monolayer culture, configuration of u03b2-cells as 3D pseudoislets enhances the GSIS response. Intact human islets' growth in both OCR and ECAR rates also increased, albeit at a smaller scale in magnitude relative to MIN6 PI. The elevated rates of glucose-stimulated ATP production in MIN6 PI were consistent with increased enzyme activity of key glycolytic and TCA cycle enzymes, as shown by the above table. There was no effect of the connexin 36 knockdown on GSIS or ATP production.
Source link: https://doi.org/10.3390/cells11152330
We investigated the effect of occludin on this metabolic transition because SIRT-1 works in conjunction with AMP-activated protein kinase. Here we show that occludin promotes AMPK expression and activation, improving glucose transporters GLUT-1 and GLUT-4, glucose uptake, and ATP content in blood-brain barrier pericytes. Perfictes also share glucose and mitochondria with astrocytes, according to our study, and occludin levels can also influence pericytes' ability to share those vital resources. In addition, we show that murine mitochondria can be transferred from live brain microvessels to energetically impaired human astrocytes, aiding their survival. Our results show that occludin plays a significant role in blood-brain barrier pericyte metabolism by influencing AMPK protein kinase activity, glucose uptake, ATP production, and banning the ability of pericytes to interact metabolically with astrocytes.
Source link: https://doi.org/10.1177/0271678x17720816
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