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Aspartic Acid - Astrophysics Data System

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Last Updated: 10 May 2022

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Differentiating aspartic acid isomers and epimers with charge transfer dissociation mass spectrometry (CTD-MS)

Charge transfer dissociation mass spectrometry aids in the identification of l- and d-epimers of Asp and isoASP amino acids in peptides. MS/MS often has trouble determining changes within LLPs, particularly when the differences are only isomeric or epimeric in nature, whereas tandem mass spectrometry in the form of collision-induced dissociation generally excels at peptide characterization. Charge transfer dissociation mass spectrometry was used to analyze spectral characteristics that could distinguish between the different isomers in this research. CTD produced diagnostic ions of the form cn+57 on the N-terminal side of iso-Asp residues, but not on the N-terminal side of Asp remains. The l- and d versions of Asp and isoAsp could also be identified based on the relative abundance of y- and z ions on the C-terminal side of Asp residues, using CTD. Differentiation was facilitated by a chiral discrimination factor, R, which compares an ion ratio in a range of one epimer or isomer to the same ion ratio in the case of a different epimer or isomer. CTD results are as reliable and statistically significant as other fragmentation techniques, such as radical directed dissociation. In summary, CTD's extensive backbone and side-chain fragments enabled the differentiation of isomers and epimers of Asp in a variety of peptides.

Source link: https://ui.adsabs.harvard.edu/abs/2022Ana...147.1159E/abstract

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions