Aquatic Animal - Europe PMC

Development and characterization of a skin cell line from Chinese perch (Siniperca chuatsi) and its application in aquatic animal viruses.

A stable and reliable cell line can play a vital role in virus identification and isolation, functional gene detection, virus pathogenic process, and antiviral immunity research. A new cell line was developed from the skin of S. chuatsi's skin in the current research. In summary, the current research provided a new tool in the study of fish viruses and its interactions with the host.

Source link: https://europepmc.org/article/MED/35762824

Development of a fully automated analytical platform based on static headspace-gas chromatography-tandem mass spectrometry for the analysis of five N-nitrosamines in dried aquatic products of animal origin.

Background To minimize the potential health risks to consumers, trace N-nitrosamines in foodstuffs' rapid and sensitive monitoring methods were needed. For the analysis of N-nitrosamines in dried aquatic products of animal origin, an analytical platform based on one-step fully automated static headspace sampling and gas chromatography-tandem mass spectrometry was developed and validated in this study. The samples and sodium chloride solution mixture were incubated in a heated headspace vial for an analyte evaporation process, as well as automatic sampling and online GC-MS/MS analysis. Relative responses in dried aquatic product samples were within a range of 76. 9-92. 4% with relative standard deviations of 1. 9-7. 2%. Conclusion These findings also demonstrated that the authenticity of the designed method for further application in trace level testing of the target analytes in foodstuffs was confirmed by the testing.

Source link: https://europepmc.org/article/MED/35704020

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