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Overall 152 patients with suspected connective tissue disorders were tested in an OPD/IPD in a tertiary care hospital in One and a half years, but only 57 out of 152 cases were screened by ANA IIF Test and ANA BLOT Test, all. ANA positivity was more apparent in females than in males. About 22 cases in the age group of 20 to 42 years showed autoimmune disease was more prevalent in the 20-40 years. According to ANA Blot, the sensitivity, specificity, positive predictive value, and negative predictive value are all positive, with a 53. 8 percent difference, compared to ANA IIF, the sensitivity, specificity, positive predictive value, and negative predictive value of ANA Blot after comparing it to ANA IIF is 77. 3 %, 87. 5 percent, 53. 8 percent, 53. 8 percent, 53. 84%, 53. 84%, 53. 8 percent, 53. 84%, 77. 3 ANA Blot Conclusion: In conclusion, indirect immunouorescence determination of ANA is the most cost-effective screening procedure for investigating autoimmune disease. ALine-Immuno Assay is used for further conrmation and detection of specic antibodies in sera, which can be quantitated with ELISA/RAC/CLIA techniques, and may help in determining prognosis, disease presence, and monitoring of therapy for a specific auto-immune disorder.
Source link: https://doi.org/10.36106/ijsr/7008538
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