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ANA Antibody - PubAg

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Last Updated: 16 October 2021

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A cashew specific monoclonal antibody recognizing the small subunit of Ana o 3

Food allergies stand for a considerable clinical responsibility and avoiding unintentional exposure to food allergens requires consistent attention. Allergic reaction to cashew nuts is regularly major, and the tiny 2S albumin, Ana o 3, is an immuno-dominant cashew irritant. In this work, a monoclonal antibody, assigned 2H5, focused on amino acids 39-- 54 within helices I and II of the tiny subunit of Ana o 3 was established that identifies both indigenous and recombinant Ana o 3 and is cashew specific in ELISA experiments. The 2H5 monoclonal anti-Ana o 3 antibody can differentiate between recombinant and indigenous healthy proteins and stands for a helpful reagent for the study of antibody cashew-allergen interactions and may make it possible for the development of cashew-specific diagnostic tools that can be utilized to avoid unintentional cashew irritant exposures.

Source link: https://pubag.nal.usda.gov/catalog/6937028


Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchos

Cathelicidin host protection peptides widely exist in animals with the functions of innate immunity and got immunity. To investigate the tissue and consecutive expression patterns of the dCATH gene, which inscribes the only cHDPs found in waterfowls, we assessed the internal body organs of healthy and balanced ducks from 1-day-old to 28-day-old raised in the native environment. The dCATH mRNA expression level across tissues was identified via measurable real-time polymerase chain response. Monoclonal antibody against the fully grown dCATH peptide used in enzyme-linked immunosorbent assay was prepared by the lymphocyte hybridoma technique for the first time. The peptide degrees of dCATH in liver, kidney, spleen, pancreas and bursa transformed with the age of duck, and the peptide concentration in these body organs was between 88 and 128 ng/g cells.

Source link: https://pubag.nal.usda.gov/catalog/7196725


Persistence of maternal antibodies to influenza A virus among captive mallards (Anas platyrhynchos)

Wild waterfowl are upkeep hosts of the majority of influenza An infection subtypes and are often the topics of IAV monitoring and transmission models. While mother's antibodies have been spotted in yolks and in nestlings for a range of wild bird types and virus, the persistence of mother's antibodies to IAVs in mallard ducklings has not been previously explored. Most of ducklings had noticeable mother's antibodies from 4 to 17 days post-hatch, while a tiny subset of individuals had observable mother's antibodies for as much as 21-33 days post-hatch. Antibody concentrations in hens near the moment of egg laying were associated with mother's antibody concentrations in the first blood example collected from ducklings. Knowledge of the duration of mother's antibodies in ducklings will help in the analysis of IAV serological surveillance results and in the modeling of IAV transmission dynamics in waterfowl.

Source link: https://pubag.nal.usda.gov/catalog/6206931


Effect of deglycosylation on immunoreactivity and in vitro pepsin digestibility of major cashew (Anacardium occidentale L.) allergen, Ana o 1

Major cashew irritant, Ana o 1, was cleansed in its indigenous form from cashew seeds and based on chemical deglycosylation using PNGase F to examine the possible role of N‐glycans in immunoreactivity. Removal of N‐glycans may have subjected formerly concealed Ana o 1 epitopes. Cleansed glycosylated and deglycosylated Ana o 1 were additionally subjected to in vitro pepsin food digestion at pH 3. 0 for 2 human resources.

Source link: https://pubag.nal.usda.gov/catalog/7309285


Prevalence of Multiple Subtypes of Avian Influenza Virus Antibodies in Egg Yolks of Mallards (Anas platyrhynchos) and White-winged Terns (Chlidonias leucopterus) in the Northeastern Republic of China

The frequencies of AIV antibodies of subtypes H1 and H3 were relatively high while the frequencies of H5 and H7 AIV subtype antibody were low. In Zhalong Wetland, the occurrence of H1 AIV subtype antibody in Mallards was the highest, with a percent of 11. 0%.

Source link: https://pubag.nal.usda.gov/catalog/6152844


Anti-DFS70 antibodies detected by immunoblot methods: A reliable tool to confirm the dense fine speckles ANA pattern

We undertook this research to evaluate the analysis performance of 2 new immunoblot methods for the detection of anti-DFS70 antibodies and to investigate whether their various DFS70 antigen structure could impact analysis accuracy in finding anti-DFS70 antibodies. 62 samples revealing a DFS70 staining pattern by IIF were tested by dot blot and line blot techniques. The results of this research reveal that there is no distinction in the overall analysis accuracy amongst approaches that make use of the trimmed or the unabridged DFS70 antigenic series which it is most likely that antibodies guided against antigens other than DFS70 may be responsible for producing a DFS70-like ANA-IIF pattern.

Source link: https://pubag.nal.usda.gov/catalog/5311033


Toxoplasma gondii Histone 4 Affects Some Functions of Murine Ana‐1 Macrophages In Vitro

Toxoplasma gondii is an obligate intracellular protozoan that can infect nearly all nucleated cells. Histone 4 is an essential element of a histone octamer. In the here and now study, T. gondii histone 4 was cloned and the governing result of TgH4 on murine macrophages was defined. Recombinant TgH4 healthy protein was identified by sera from rats experimentally infected with T. gondii and indigenous TgH4 in the complete soluble healthy protein of T. gondii tachyzoites was identified by polyclonal antibodies versus rTgH4, as shown by immunoblotting evaluation. Viewed together, these results showed that rTgH4 can influence the functions of murine macrophages in vitro.

Source link: https://pubag.nal.usda.gov/catalog/6199882


Ecological determinants of avian influenza virus, west nile virus, and avian paramyxovirus infection and antibody status in blue-winged teal (anas discors) in the canadian prairies

Thousands of countless waterfowl of countless types from numerous flyways merge in and disperse from this area each year; as a result this area may be a vital area for potential intra- and interspecific spread of transmittable microorganisms among migratory waterfowl in the Americas. Making Use Of Blue-winged Teal, which have one of the most considerable migratory variety among waterfowl types, we explored ecologic risk variables for infection and antibody status to avian flu infection, West Nile virus, and avian paramyxovirus-1 in the 3 grassy field provinces prior to fall movement. The probability of AIV infection in BWTE was associated with host aspects, population-level aspects, and year. An interaction in between age and AIV antibody status revealed that hatch year birds with antibodies to AIV were a lot more likely to be infected, recommending an antibody response to an active infection.

Source link: https://pubag.nal.usda.gov/catalog/4781191


Antibodies to H5 subtype avian influenza virus and Japanese encephalitis virus in northern pintails (Anas acuta) sampled in Japan

Antibodies to JEV and WNV were spotted in 5 and none of the examples, respectively. Outcomes provide evidence for previous exposure of migrating northern pintails to H5 AIV which can have ramifications for viral shedding and illness occurrence.

Source link: https://pubag.nal.usda.gov/catalog/57843


The identification, characterization, and function of two TREMs genes in Chinese Yangzhou goose (Anas cygnoides)

Triggering receptor expressed on myeloid cells is a cell-surface receptor mostly shared on macrophages. AcTREM1 expression in the liver and spleen was considerably upregulated complying with lipopolysaccharide difficulty at an early stage of infection and after that decreased at a later phase. Changes in AcTREM2 expression were reciprocatory to those of AcTREM1 in the liver and spleen after LPS difficulty. Our results suggest that AcTREM1 and AcTREM2 take part in the antibacterial immunity of A. cygnoides.

Source link: https://pubag.nal.usda.gov/catalog/5657428

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions