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Objectives: We wanted to know the prevalence of amoebic infection in Sulaymaniyah province and testing of certain immunological parameters in amoebic infected children. Results: The total prevalence of amoebic infection was a male, while females had a higher incidence, although female had a higher incidence of amoebic disease. The patients who used general tap water for drinking had the highest rate of infection in September, October, and then November were the most common bacteriae borne diseases, with no change in between. In those children whose mother was illiterate, the highest amoebic infection rate was discovered. serum levels of IL-17 were not significantly different between infected children and control groups; however, the IFN level was reported to be highly different; however, TNF- serum level was found to be highly significant; however, TNF- serum concentrations in IL-17 were not significantly different between control groups and control groups;however, IFN levels were not significantly different; however, TNF- serum levels were not significantly different; however, TNF- serum levels remained unchanged Conclusion: Based on the microscopic diagnosis, we found that the amoebic infection prevalence in Sulaymaniyah province was highest among symptomatic children.
Source link: https://doi.org/10.22317/imj.v6i1.1148
We hypothesize that L. pneumophila growth in the atmosphere may be partially dependent on amoebic populations' composition and potential interactions between various amoebic species. With A. castellanii and W. magna being able to ingest V. vermiformis infected or not infected with L. pneumophila, we show the occurrence of inter-amoebic phagocytosis. We also found that L. pneumophila growth is largely affected by each interactive amoeba's, which shows that L. pneumophila growth and spread are largely controlled by inter-amoebic interactions, at least in part.
Source link: https://doi.org/10.1093/femsle/fnaa147
mNGS also found 42,899 and 1,337 specific sequences of N. fowleri in the CSF and blood samples, respectively. The mNGS detection was confirmed by PCR results and pathological smear later. Primary amoebic meningoencephalitis was finally diagnosed in the patient. Moreover, 15 similar child infection cases in the last ten years were reviewed and analyzed in this article to promote the early diagnosis of this rare disease.
Source link: https://doi.org/10.3389/fped.2022.785735
Plants of Tabernaemontana species have many pharmacological functions, including antimicrobial activity. Amoebiasis remains a public health issue, despite growing evidence of resistance to metronidazole. Entamoeba histolytica trophozoites' viability and infectivity were determined by an alkaloid fraction of T. arborea root bark and its alkaloids ibogaine and voacangine in this research. The alkaloid fraction, but mostly ibogaine and voacangine alkaloids, showed potent dose-dependent anti-amoebic activity at 24h post-exposure, but not like metronidazole. Significant morphological changes in the trophozoites, marginalization rise, and death by apoptosis were induced by the treatment of trophozoites in the IC50s for 24 h. T. arborea alkaloids' ability was tested to reduce the incidence of amoebic liver absces in hamsters.
Source link: https://doi.org/10.1055/a-1809-1157
With four separate real-time PCR assays, nine ATCC strains of Acanthamoeba and 40 delinked, biobanked, surplus corneal scraping samples were tested for the presence of acanthamoebae, contributing to acanthamoebae and 40 delinked, biobanked, surplus corneal scraping specimens were tested for acanthamoebae. The assay used by the Free-Living and Intestinal Amebas Laboratory of the CDC was considered the reference standard, and its results were determined by each individual assay and pair of assays. Acanthamoeba's that could not be detected by some individual assays included Acanthamoeba castellanii, Acanthamoeba culbertsoni, and Acanthamoeba lenticulata. False assay of the gold standard assay could not be excluded from three clinical samples. Molecular diagnostic approaches, particularly combinations of highly sensitive and specific assays, are likely to be more cost-effective in clinical specimens and more cost-effective than either culture or direct microscopic examination.
Source link: https://doi.org/10.1128/jcm.01607-15
Entamoeba histolytica is not a common causative agent of acute appendicitis, according to the study. However, amoebic appendicitis can sometimes be painful and life threatening, owing to a lack of knowledge. The study participants were HIV-1-infected individuals with acute appendicitis and then underwent appendectomy at our hospital between 1996 and 2014. In 57 patients with acute appendicitis, appendectomies were administered. E. histolytica was 33% from the previously stored sera, according to his seropreval. In the present study, E. histolytica infection was found in 9 cases. There were no other differences in the clinical characteristics of the PCR-positive and -negative groups apart from a significantly higher leukocyte count in E. histolytica -positive patients rather than in negative patients. In nine of the appendicitis cases, E. histolytica infection was confirmed in 9.
Source link: https://doi.org/10.1128/jcm.01757-16
ABSTRACT TRACT AMoebic keratitis causes significant ocular morbidity in contact lens wearers. Amoebic keratitis diagnostic methods are highly sensitive, labor-intensive, and have a slow turnaround time. In clinical specimens, we analyzed four laboratory methods for the detection of acanthamoebae. Deidentified, delinked consecutive specimens from patients with suspected amoebic keratitis were assayed for acanthamoebae by prompt smear analysis, culture, and PCR using two different primer sets developed for Acanthamoeba ribosomal DNA using two different primer sets. A single-organism level of analytic sensitivity was demonstrated by a Nelson primer PCR. These results are based on small numbers and should be treated cautiously, owing to sample quality, with contact lenses and casings showing the highest detection rates of detectable acanthamoebae and the highest diagnostic sensibilities for direct smear measurement, culture, and JDP primer PCR, but these findings are not limited and should be interpreted cautiously.
Source link: https://doi.org/10.1128/jcm.00173-09
In the gerbil model of ALA, Gerbils were vaccinated intramuscularly with cytosine oligodeoxynucleotide as adjuvant to raise Th1 responses against Gal-lectin, and control gerbils received CpG-ODN alone. When CpG-ODN was used as adjuvant and Gal-lectin, there was clearly protected against the onset of ALA, with 65% of controls developing ALA and 85% of controls displaying ALA. CpG-ODN and Gal-lectin vaccine recipients who were vaccinated with CpG-ODN and Gal-lectin also had elevated gamma interferon, interleukin-12, and IL-2 mRNA than controls. This is the first review of the use of CpG as a potent Th1 adjuvant with Gal-lectin to shield against ALA formation.
Source link: https://doi.org/10.1128/iai.74.1.528-536.2006
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