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ACYL CARRIER PROTEIN - Crossref

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Last Updated: 10 August 2022

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Specialized acyl carrier protein used by serine palmitoyltransferase to synthesize sphingolipids in Rhodobacteria

Serine palmitoyltransferase catalyzes the first and committed step in sphingolipid biosynthesis, condensating L-serine and acyl-CoA to form 3-oxo-sphinganine. If the structural gene for SPT is present in genomes of Rhodobacteria, it co-occurs with genes coding for a putative acyl carrier protein and a putative acyl synthetase. Here we demonstrate that the putative ACP's 4'-phosphopantetheine prosthetic group is selectively acylated by the ACS, ensuring the provision of a specialized ACP for Rhodobacteria synthesis. SPTs from C. crescentus, Escherichia coli B, or Sphingomonas wittichii can be used preferentially acyl-AcpR as a thioester substrate for 3-oxo-sphinganine synthesis. Whereas acyl-AcpR from C. crescentus is a good substrate for SPTs from distinct Rhodobacteria, acylation of a particular AcpR is achieved by the cognate AasR from the same bacterium. Rhodobacteria may rely on this more subtle method of 3-oxo-sphinganine formation to promote free fatty acids toward sphingolipid biosynthesis.

Source link: https://doi.org/10.3389/fmicb.2022.961041


Identification of Novel Inhibitor of Enoyl-Acyl Carrier Protein Reductase (InhA) Enzyme in Mycobacterium tuberculosis from Plant-Derived Metabolites: An In Silico Study

Mycobacterium tuberculosis enoyl carrier protein reductase has been identified as a useful target for tuberculosis therapy, and is considered a promising agent for drug discovery. Pharmacokinetics and molecular docking results were compiled by a team of Ruta graveolens and citrus plants belonging to the Rutaceae family. The target protein's chemical compositions and the 3D structure of the target protein were discovered from the PubChem database and RCSB Protein Data Bank, respectively. Against the InhA target, the binding Efficacy of gravacridonediol was higher than that of the well-known inhibitor triclosan. The present review shows that the identified natural compound gravacridonediol has drug-like characteristics as well as a promise in blocking InhA, a key target enzyme of M. tb.

Source link: https://doi.org/10.3390/antibiotics11081038


Acyl Carrier Protein is essential for MukBEF action in Escherichia coli chromosome organization-segregation

Essential accessory proteins, kleisins that link an SMC dimer's two subunits, and HAWK/KITE accessory proteins that work with kleisins are among the Dimeric SMC proteins' key cleisins. We also show that MukB interacts with Acyl Carrier Protein, which has essential functions in fatty acid synthesis, here. We describe AcpP's liaison site at the MukB coiled-coil's joint, which indicates that it is vital for MukB ATPase and MukBEF operation in vivo.

Source link: https://doi.org/10.21203/rs.3.rs-547787/v1


Structural Comparison of Acinetobacter baumannii β-ketoacyl-acyl Carrier Protein Reductases in Fatty Acid and Aryl Polyene Biosynthesis

APE biosynthesis gene clusters for APE biosynthesis have striking similarities with fatty acid synthesis genes. Despite the fact that the widespread distribution of pathogenic and symbiotic bacteria is unclear, the specific roles of APE gene clusters' metabolic products are uncertain. We investigated the crystal structures of the u03b2-acyl carrier protein reductase ApeQ generated by an APE gene cluster from clinically isolated virulent Acinetobacter baumannii in two states here. The APE polyene FabG from the A. baumannii FAS gene cluster's in vitro visible absorption spectrum assay of the APE polyene moiety could not be substituted for ApeQ in APE biosynthesis.

Source link: https://doi.org/10.21203/rs.3.rs-107949/v1


Engineering the β-ketoacyl-acyl carrier protein synthase gene in the synthesis of cell wall mycolic acids in Mycobacterium neoaurum for enhancing the bioconversion of steroidal intermediates from phytosterols

Abstract Background: The conversion of phytosterols into high-value-added steroidal intermediates is the keystone of the steroid pharmaceutical industry. In this research, a robust and stable modification of the cell wall dramatically improved the cell wall's permeability for the increased production of steroids. In the vegetative cell transformation, the yield of 9 u03b1-hydroxy-4-androstene-3,17-dione was up by 137. 7%. Ultimately, the 9-OHAD production in an industrial used resting cell system had hit 0. 1135 g/h and the conversion time was reduced by 33%. Conclusions : The modification of kasB resulted in a significant change in the cell wall mycolic acids.

Source link: https://doi.org/10.21203/rs.2.19378/v1


New insights on the function of plant acyl carrier proteins from comparative and evolutionary analysis

Abstract Background Acyl carrier proteins play a significant role in plant cell synthesis of both plastidial and mitochondrial Type II fatty acid synthesis. A small number of ACP genes undergo tandem or segmental duplication, corresponding to two rounds of whole genome duplication. According to the Ka/Ks ratios, duplicated ACP genes underwent a purifying selection process. Notably, the average expression levels of ACP1, mtACP3, and mtACP1 were positively correlated with those of ACP3, ACP4, and mtACP2, respectively. Seven motifs related to tissue-specific, ABA, and light-mediated gene regulation were present in all ACPs tested, leading to new insight into the FAS' central regulatory enzymatic partners. Detailed information on angiosperm ACP gene families is included in this report, as well as insightful recommendations for the functional analysis of plant ACP gene families in the future.

Source link: https://doi.org/10.21203/rs.2.18739/v1


Involvement of Arabidopsis Acyl Carrier Protein 1 in PAMP-Triggered Immunity

Plant fatty acids and lipids are both essential in storing electricity and acting as structural components for cell membranes and signaling molecules for plant growth and stress responses. During FAs' elongation, Acyl carrier proteins are small acidic proteins that covalently attach the fatty acyl intermediates. The Arabidopsis thaliana ACP family has eight members. acp1 mutant plants also have a higher level of SA and show corresponding shifts in JA- and SA-regulated transcriptional outputs, which is consistent with the established antagonistic relationship between JA and salicylic acid. In addition, methyl JA and linolenic acid treatments have been reported to have an apparently reduced risk of resistance against P. syringae pv. This tomato in acp1 mutants is more prolific in acp1 mutants than in WT plants, and it's not in WT plants. According to ACP1, FA metabolism and hormone homeostasis can be negatively involved in PTI in Arabidopsis plant defense.

Source link: https://doi.org/10.1094/mpmi-02-22-0049-r

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

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* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions