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ACT Prep - Crossref

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Last Updated: 10 May 2022

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Bone changes with candidate PrEP regimens containing tenofovir disoproxil fumarate and/or maraviroc and/or emtricitabine in US men and women: HPTN 069/ACTG A5305

The bone mineral density decreases have been linked to decreases in bone mineral density, according to Tenofovir disoproxil fumarate-containing pre-exposure prophylaxis, but other non-tenofovir disoproxil fumarate candidate PrEP regimens are not well defined. Methods The HPTN 069/ACTG A5305 study randomized 406 U. S. cisgender men and transgender people, as well as 188 cisgender people at risk of HIV infection to one of four double-blinded regimens, including maraviroc + emtricitabine, gygoriacetate; maraviroc + emtricitabine; or tenofovir disoproxil fumarate The change in LS and hip BMD measured by Wilcoxon rank-sum testing and multiple linear regression adjusted for sex, race, and baseline BMI was compared between the tenofovir disoproxil fumarate-containing arms and non-tenofovir disoproxil fumarate-containing arms. The average percentage change in BMD in the tenofovir fumarate arms at 48 weeks was – 1. 5% in the tenofovir disoproxil fumarate arms and 0. 0% in the non-tenofovir fumarate arms at the hip.

Source link: https://doi.org/10.1093/jac/dkab400


DNA extraction and Nanopore library prep from 15-30 whole flies v1

We've been gathering the genomes of many Drosophila species. We created this guide to keep the cost of sequencing down to $500 per assembly while still having a large number of very long reads. A typical Drosophila Nanopore sequencing run should have read N50 of 20-40kbp with 5-15% of sample reads > 100kbp if using these instructions. We usually see 3-4 species per 2 flow cells, with mainly for 14-18 Gbp of data per flow cell.

Source link: https://doi.org/10.17504/protocols.io.bdfqi3mw


DNA extraction and Nanopore library prep from single wild-caught drosophilids v1

In comparison to our previous method that used gDNA extracted from tens or hundreds of flies from an inbred line, this protocol fork allows for the sequencing of single wild-caught flies using Nanopore MinION sequencers. Although read lengths are not nearly identical to what we were seeing before, libraries range from 2-10kb read N50 to custom genome assemblies with contig N50 > 1 Mbp.

Source link: https://doi.org/10.17504/protocols.io.b7r9rm96


DNA extraction and Nanopore library prep from single wild-caught drosophilids v2

This protocol fork allows for the sequencing of single wild-caught flies using Nanopore MinION sequencers, in comparison to our previous procedure that used gDNA extracted from tens or hundreds of flies from an inbred line. Although read lengths aren't exactly similar to what we were seeing before, library collections ranging from 2-10kb read N50 to commonly produced genome assemblies with contig N50 > 1 Mbp.

Source link: https://doi.org/10.17504/protocols.io.b7wprpdn


FloodLAMP Inactivation Solution Prep v1.0 v1

The TCEP should be made in a chemical fume hood as the TCEP is caustic. If yellow Binding Solution does not go further than an inactivated sample or PBS+Inactivation Soln, you may have degraded, and repeat with another aliquot or newly produced Inactivation Kit. Albert Yu of Brandeis has also suggested that instead of 1. 15N NaOH, the salivary glands be used in a 1. 4N NaOH solution. NaOH should be scaled up and water down by 1. 4/1. 15 and water down for this.

Source link: https://doi.org/10.17504/protocols.io.bk5qky5w


Characterizing HIV-preventive, plasma tenofovir concentrations. A pooled participant-level data analysis from HIV pre-exposure prophylaxis (PrEP) clinical trials.

Abstract Background The daily dosing of tenofovir disoproxil fumarate, with or without emtricitabine, has a high rate of HIV prevention in those people are adherent. However, the tenofovir target protective plasma concentration is not entirely understood. The aim of this research is to determine the protective TFV plasma concentrations. Following oral administration, the estimated protective TFV trough concentration in high risk females was markedly higher in high risk females compared to high risk males and to low risk individuals. According to Dosing studies, high-risk women require full cooperation to maintain their protection levels. Conclusions We created a population adherence-PK-risk-outcome calculator using the largest PK-HIV result database to date. According to our findings, females at a higher risk of HIV vaccination need greater plasma TFV to achieve HIV protection than males. If daily adherence is maintained, HIV protection in all populations hits 90%, with 90 percent in all populations.

Source link: https://doi.org/10.1093/cid/ciac313


Preferences for Long-Acting and Alternative Modalities for PrEP among Military Men Who Have Sex with Men: Segmentation Results of an Adaptive Choice-Based Conjoint Analysis Study

Abstract The use of pre-exposure prophylaxis for HIV prevention in the United States military is poor. Implementing preference-based alternative delivery methods of PrEP delivery, on the other hand, could be a pioneering measure to crack the specific barriers to PrEP enrollment among military MSM. The anonymous, adaptive choice-based conjoint analysis, which identified five key characteristics of concern, found five key points of concern among HIV-negative military men who have sex with men. Changes in program interest rates among segments were then used to investigate shifts in program interest rates among segments by market simulation. When smartphone, civilian-based, and long-acting injectable PrEP options were involved, Simulations revealed that PrEP program interest among two segments with low interest rates soared. Both responding to segment-specific needs in the development of military PrEP programs and emphasizing the importance of clinical relationships within the context of PrEP involvement in a military environment may contribute to increased PrEP uptake.

Source link: https://doi.org/10.1007/s11524-022-00615-9


DNA extraction and Nanopore library prep from single wild-caught drosophilids v2

This protocol fork allows for the sequencing of single wild-caught flies using Nanopore MinION sequencers, in comparison to the previous method that used gDNA extracted from tens or hundreds of flies from an inbred line. Though read lengths aren't exactly like what we were seeing before, librarians can vary from 2-10 Kb read N50 to generally produce genome assemblies with contig N50 > 1 Mbp.

Source link: https://doi.org/10.17504/protocols.io.dm6gpbdn8lzp/v2


HIV Prevention During the COVID-19 Pandemic: Sexual Activity and PrEP Use Among Black Same-Gender-Loving Men and Black Cisgender Women

We conducted 20 in-depth interviews—12 with Black same-gender men and 8 with Black cisgender people and 8 with Black cisgender women—to explore the effects of the COVID-19 pandemic on sexual development and PrEP use in the initial phase. Both groups were worried about contracting COVID-19 through sexual partners, while males described selecting partners based on perceived COVID-19 risk. Future qualitative research is required to figure out how sexual habits and PrEP use changed as the pandemic epidemic progressed.

Source link: https://doi.org/10.1521/aeap.2022.34.2.142

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions