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3T3 Cell - Crossref

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Last Updated: 14 June 2022

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AG490 inhibits G1-S traverse in BALB/c-3T3 cells following either mitogenic stimulation or exogenous expression of E2F-1

"While early STAT gene expression was repressed by AG490, extracellular signal-regulated kinase activation was unaffected, and a pattern of gene expression showed cells exited G0 in the absence of the inhibitor. " Although AG490 did not change the induction of cyclin D1 protein, no cyclin D1- nor cyclin D3-associated kinase activity was detected in growth-inhibited cells. These results indicate that AG490 has blocked a cellular pathway required for mid-G0-G1 traverse, which is located after the induction of early processes that could have been mediated by E2F but not until the late G1 surge in E2F-dependent transcription. Induction of cyclin A by an E2F-1 adenovirus infection in AG490-treated cells led to the release of cyclin A, but it was unable to recover from the drug-induced cell cycle arrest that was coincident with the suppression of cyclin-dependent kinase 2 kinase activation. ".

Source link: https://doi.org/10.1158/1535-7163.205.3.2


MiR-185 inhibits 3T3-L1 cell differentiation by targeting SREBP - 1

"During 3T3-L1 cells differentiation, the miR-185 expression gradually increased during this research. " The protein level of PPARu03b3, FABP4, FAS, and LPL were dramatically reduced by overexpression of miR-185, PPARu03b3, FABP4, and LPL, as well as the protein level of PPARu03b3 and FAS. We established that sterol regulatory element binding protein 1 may be the target of miR-185 by target gene expression and luciferase reporter assay. MiR-185 negatively regulates 3T3-L1 cells differentiation by focusing on SREBP-1, thus undermining the role of miRNAs in adipogenesis. ".

Source link: https://doi.org/10.1080/09168451.2017.1347485


Isolation of phenolic acids and tannin acids from Mangifera indica L. kernels as inhibitors of lipid accumulation in 3T3-L1 cells

"ABSTRACT Mango kernels are generally discarded as garbage, but they do contain many pharmacological characteristics and bioactivities," says the author. In 3T3-L1 adipocytes, we isolated antiobesity agents from mango kernels that inhibit intracellular lipid formation. In addition, ethyl digallate, PGG, and HGG all negatively reduced the mRNA expression of adipogenic transcription factors such as C/EBP u03b1 and PPARu03b3. However, ethyl gallate did not influence the expression of these transcription factors," "these transcription factors did not influence the expression of these transcription factors. ".

Source link: https://doi.org/10.1093/bbb/zbac030


Deficient of LRRC8A attenuates hypoxia-induced necrosis in 3T3-L1 cells

"ABSTRACT HEARTLY is a film that appears under acute hypoxia Multiple ion channels on the cell membrane are activated, resulting in cell swelling and eventually necrosis, leading to cell swelling and eventually necrosis. " The deposition of LR8A in 3T3-L1 cells was also attributed to a significant anti-necrotic phenotype of hypoxia and the reduction of necrosis-related genes. "In vivo, the difference in the LRRC8 family members was also identified between high-altitude pigs and their low-altitude relatives. ".

Source link: https://doi.org/10.1080/09168451.2020.1730689


Suppression of NSDHL attenuates adipogenesis with a downregulation of LXR-SREBP1 pathway in 3T3-L1 cells

"Abstract" is a word that appears different during 3T3-L1 differentiation, but its roles in adipogenesis are unknown. " In NSDHL-knockdown 3T3-L1 cells, the expression peak of NSDHL in the early stages of 3T3-L1 preadipocyte differentiation and LXR-SREBP1 signaling pathway was downregulated, according to further studies.

Source link: https://doi.org/10.1080/09168451.2020.1719823


Influence of Antiestrogens on NIH-3T3-Fibroblast-Induced Motility of Breast Cancer Cells

"In some instances, stromal tissue surrounding the tumor cells supplies these growth factors that interact with tumor cells and therefore may play a significant role in tumor formation and progression. " The invasiveness of breast cancer cells has been shown by conditioned media from NIH 3T3 fibroblasts, according to We and others. The present research investigated the effects of antit3-CM on breast cancer cell motility and found that antiestrogens minimized this 3T3-CM-induced phenomenon. MCF-7 cells' 3T3-CM-induced scattering motility has been largely responsible for the 3T3-CM-induced scattering motility of 3T3-CM cells, according to the findings of this study. These findings illustrate the importance of stromal-tumor cell interactions in breast cancer cell motility. Antiestrogens reduce breast cancer cell motility and breast cancer cell motility, according to these findings, and antiestrogen therapy can be used to reduce breast cancer cell motility and breast cancer cell motility.

Source link: https://doi.org/10.1159/000048502


Water and Ethanol Extracts of Tamarind (Tamarindus indica) Suppress Lipid Accumulation in 3T3-L1 Cells

"A new in vivo report showed that tamarind may reduce the bodyweight of obese rats. " Both tamarind water and ethanol extracts had non-cytotoxicity activity on 3T3-L1 cells, according to the study. In addition, tamarind water extract at 10 mg/mL reduced the total lipid content by 85% relative to that of control, with less lipid lowering capability at all doses tested. This research revealed that tamarind water extract had the ability to reduce lipid accumulation, reducing lipid accumulation, thus giving an anti-obesity result. ".

Source link: https://doi.org/10.20956/canrea.v5i1.630


Differential effects of novel tumour-derived p53 mutations on the transformation of NIH-3T3 cells

"Abstract" (Abstract): "The p53 tumour suppressor gene is frequently altered in human tumors and some tumor-derived mutations have a variety of effects on cells. " After irradiation and response to chemotherapeutic drugs, the effects of a novel tumor-derived p53 mutation as well as two recently reported mutations from South African breast cancer patients was investigated. Cells expressing the p53 mutants were either more sensitive to cisplatin and melphalan or more resistant than the untransfected cells, depending on the mutation. These results reveal that different p53 mutations have different biological effects on normal cells, with some altering checkpoint activation more quickly than others. The genesis of the p53 mutation, according to the results, also influences the sensitivity to cytostatic drugs. ".

Source link: https://doi.org/10.1515/bc.2008.010


Effects of co-incubation of LPS-stimulated RAW 264.7 macrophages on leptin production by 3T3-L1 adipocytes: A method for co-incubating distinct adipose tissue cell lines

"Inflammatory adipokine release is attributed to the changes in adipose tissue in the overfed state, where tissue remodeling has led to tissue destruction in hypertrophic adipocytes that recruit monocytes, which contributes to tissue infiltration and has an inflammatory phenotype. " As the number of inflammatory macrophages rises, an increase in the production of macrophage-specific inflammatory mediators is also present, contributing to the inflammatory state and establishing a protective loop between the macrophages and adipocytes. Although most inflammatory adipokines are released by macrophages, adipocytes can also produce immunomodulatory adipokines, such as leptin. In wells, we noticed increased amounts of leptin relative to Transwells. Adipocytes had a poor negative association between nitric oxide release by macrophages and leptin release by adipocytes.

Source link: https://doi.org/10.1101/2021.07.27.454005

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions