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Abstract Cancer, one of the most common health issues, remains unsolved. We're interested in synthesising and evaluating antitumor activity of spiro[2,1-b]pyridine[2,3-d] and spiro[2,1-b]pyrimidine] and spiro[2,3-d]pyrimidine] derivatives in this regard. To determine the biological activity of our compounds, we used 2D and 3D cell culture models. The compounds 4a and 4e are the best among our seven products, with a 50% growth inhibitory concentration value lower than etoposide, according to our results. Our findings support the claim that the products 4a and 4e could be useful for drug design in cancer therapy.
Source link: https://doi.org/10.1007/s11030-022-10378-9
The importance of 3D cell cultureu2014i. e. , cell stability by mimicking the natural environmentu2014 has been on the rise, and various 3D cell culture techniques have been developed. A commonly used cell culture technique, 3D cell culture, is a common form of matrix-free 3D cell culture. Cells aggregat in a spherical shape with abundant cellu2013cell and cellu2013matrix interactions that more closely mimic the in vivo environment in a 3D culture, unlike those in traditional 2D cell culture.
Source link: https://doi.org/10.1007/s13206-022-00086-9
Lung cancer is the most common cause of cancer deaths globally, with the majority of the reported cases related to non-small cell lung cancer. Nisin ZP is a polycyclic antimicrobial peptide produced by the Gram-positive bacterium Lactococcus lactis and is commonly used as a food preservative. Nisin ZP has recently reported anticancer activity in melanoma, head and neck squamous cell carcinoma, hepatic, colon, and blood cancer. In this research, we investigated the anticancer activity of nisin ZP and determined the underlying mechanisms in NSCLC cells. After 48 hours of therapy, the findings revealed that nisin ZP induced selective toxicity in cancer cells relative to healthy cells. Regardless of tumor protein p53 expression, Nisin ZP exposure induced apoptosis and cell cycle arrest in NSCLC cells. Compared to placebo, nisin ZP inhibited the A549 cells' 3D spheroid growth and cell viability significantly impeded cell growth and cell viability.
Source link: https://doi.org/10.1007/s11095-022-03220-2
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