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3D Cell Culture - Europe PMC

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Last Updated: 10 August 2022

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Three-dimensional (3D) cell culture: a valuable step in advancing treatments for human hepatocellular carcinoma.

Hepatocellular carcinoma is the fifth most common malignant tumor and the third most common cause of tumour-related deaths worldwide. Many surgical and medical therapeutic options are currently available for HCCs, including chemotherapy and immunoglobal cells within the tumour microenvironment; however, the interaction between neoplastic cells and non-neoplastic stromal cells within the tumour microenvironment leads to significant therapeutic resistance to HCCs to conventional therapy. In vivo and vitro, a three-dimensional culture model could be a crucial bridge between in vivo and vitro due to its ability to imitate the naturally complex in vivo TME compared to traditional two-dimensional cultures.

Source link: https://europepmc.org/article/MED/35908054


Vitamin D 3 and Salinomycin synergy in MCF-7 cells cause cell death via endoplasmic reticulum stress in monolayer and 3D cell culture.

In several cancer cell lines in vitro, dihydroxyvitamin D 3, the active form of vitamin D3, has antitumor activity in 25 cell lines. Salinomycin has anticancerogenic activity against cancer cell lines. This research aims to determine the cytotoxic and antiproliferative effects of Sal associated with 1,25D on MCF-7 breast carcinoma cell lines cultured in monolayer and three-dimensional models. Overall, Sal and 1,25D act synergistically, preventing cell proliferation by stimulating simultaneously multiple death pathways and may be a novel and promising luminal A breast cancer treatment program.

Source link: https://europepmc.org/article/MED/35914560


Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes.

In this research, we developed a microfluidic large-scale integration chip platform to facilitate the generation, long-term culture, and extraction of 3D adipose microtissues from 3D adipose microtissues in vitro. Human adipose stem cells differentiated into mature adipocytes in 32 individual fluidically accessible cell culture chambers, which were developed to promote three microtissue differentiation and cell culture. We determined the minimum cell numbers required to obtain robust and complex proteomes with over 1800 identified proteins by coupling mass spectrometry to the cell culture platform. Every 6 h for the course of a week, the adipose microtissues on the chip platform were used to occasionally simulate food intake by alternating the glucose content in the cell-feeding media every 6 h.

Source link: https://europepmc.org/article/MED/35875914


Lipid droplets and perilipins in canine osteosarcoma. Investigations on tumor tissue, 2D and 3D cell culture models.

This paper, which focuses on lipid droplet-coating proteins, aims to shed light on the presence and development of lipid droplets in canine osteosarcoma. Canine osteosarcoma tissue samples were analyzed for lipid droplets and lipid droplet-coating proteins by immunohistochemistry and electron microscopy for lipid droplets and lipid droplet-coating proteins for this purpose. In addition, we used the canine osteosarcoma cell lines D-17 and COS4288 in 2D monolayer and 3D spheroid in vitro models, and then analyzed the samples by means of histochemistry, immunofluorescence, molecular biological methods, and electron microscopical imaging. A increase of lipid droplet amount was observed alongside an increase in PLIN2 expression as a result of external lipid supplementation. Detailed electron microscopical investigation revealed that lipid droplet sizes in tumor tissues were comparable to those in 3D spheroid models. In conclusion, the 3D spheroids can be used as a useful in vitro model for further studies on lipid droplets biology and function in osteosarcoma.

Source link: https://europepmc.org/article/MED/35834072


3D printed devices with integrated collagen scaffolds for cell culture studies including transepithelial/transendothelial electrical resistance (TEER) measurements.

To produce the collagen scaffold, Electrospinning was used to create the collagen scaffold, followed by an optimized 1-Ethyl-3-carbodiimide/N-Hydroxysuccinimide cross-linking process to produce stable collagen fibers that are similar in shape to fibers in vivo. The leaching of solvent and NHS from the scaffold was investigated by an LC/MS, with several rinsing steps being taken to avoid leaching and promote the culture of Madin-Darby Canine Kidney epithelial cells on the scaffold. For cell culture, a comparative analysis was made between collagen scaffolding and other electrospun materials. Overall, this study reveals that the combination of biological ECM components such as collagen and microfluidic devices that incorporate flow has the ability to produce more accurate cell culture models in fields such as blood brain barrier research.

Source link: https://europepmc.org/article/MED/35934386

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions