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"Results" We found a one-step conversion of sucrose obtained from cyanobacteria to fine chemicals by constructing a microbial consortium containing the fast-growing cyanobacterium Synechobacterium Synechobacteria elongatus 304 and Escherichia coli to produce sucrose and then the platform chemical 3-hydroxypropionic acid from CO2 under photoautotrophic growth conditions. Under the strong promoter P cpc560 in S. elongatus UTEX 2973 for effective sucrose secretion, efforts were made to overexpress the sucrose permease-coding gene cscB. Cell growth of S. elongatus UTEX 2973 in the consortium was also increased, perhaps as a result of the rapid quenching of reactive oxygen species in the system by E. coli, which in turn improved the photosynthesis of cyanobacteria. The study also showed that heterotrophic bacteria could promote cyanobacteria cell growth by lowering oxidative stress in this microbial community, which further increases the potential value of this system for future industrial applications. ".
Source link: https://doi.org/10.1186/s13068-020-01720-0
"Abstract Background 3-Hydroxypropionic acid is a common platform chemical that can be obtained chemically from glycerol. Klebsiella pneumoniae, an ideal biocatalyst for 3-HP because it can grow well on glycerol and then synthesize the essential coenzyme B12 in a natural biocatalyst. If higher yields and titers of 3-HP are to be achieved, the continued improvement of NAD+ under anaerobic environment, in which coenzyme B12 is synthesized sustainably is required. Results In this study, recombinant K. pneumoniae L17 overexpressing aldehyde dehydrogenase was produced and cultured in a bioelectrochemical system, with the addition of an electrical potential to the anode using a chronometerometric method. When the L17 cells were grown under an electric potential, the intracellular NADH/NAD+ ratio was significantly lower. Conclusions Enhanced 3-HP production by the BES was made possible by using recombinant K. pneumoniae L17. Experimental respiratory uncoupler experiments investigated the electrode-to-L17 transitionence between the electrode and L17.
Source link: https://doi.org/10.1186/s13068-017-0886-x
MCT specifically promoted the reversible change of mesaconyl-CoA to mesaconyl-C4-CoA, a key reaction intermediate for carbon fixation, "Different from the well-known CoA transferases that catalyze CoA movement between two distinct substrates, MCT specifically catalyzes CoA exchange between two distinct substrates. Here we established the crystal structure of MCT from a chlorosome-less FAP Roseiflexus castenholzii at 2. 5 u00c5 resolution, and characterized the catalytic mechanisms by structural analysis and molecular dynamic simulations. R. castenholzi MCT's main website and a small domain that are linked by two linkers are connected by two linkers, owing to a Rossmann fold larger domain and a small domain that are linked by two links. In the case, where the substrate binding pockets are located at the interface of the Rossmann fold larger domain from one subunit and the small domain from the other subunit, two MCT subunits are cross interlocked at the linker regions to create a functional dimer in solution, the substrate binding pockets are located at the interface of the Rossmann fold larger domain from one subunit and the small domain from the other subunit. ".
Source link: https://doi.org/10.3389/fmicb.2022.923367
"3-Hydroxypropionic acid is a promising high value-added product, and it is a popular high value-added product. " In the present study, we developed a strategy to minimize Acc toxicity cell growth by Acc subunit expression adjustment, which was a solution to these problems. The engineered strain had the highest titer of 6. 8 g/L with a high yield of 0. 566 g/L/h and high yield of 0. 13 g/L/h during shake-flask fermentation via the malonyl-CoA pathway but was also used for 3-HP manufacturing later in a shake-flask fermentation.
Source link: https://doi.org/10.3389/fmicb.2022.902848
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