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16S rRNA Sequencing - DOAJ

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Last Updated: 10 June 2022

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Evaluating Starter Feeding on Ruminal Function in Yak Calves: Combined 16S rRNA Sequencing and Metabolomics

"Start feeding can effectively promote rumen growth and development in ruminants for young ruminants," says the narrator, but rumen formation is a significant physiological challenge, considering that the mechanism of starter feeding stimulating remains unclear. " In this research, we did an investigation of ruminal microbiota and their metabolites in yak calves to see how the ruminal microbiota and their metabolites promote ruminal function. In yak calves, this research used 16S rRNA sequencing with liquid chromatography-mass spectrometry to determine the effects of starter feeding on ruminal microbiota diversity and metabolites. We conducted the experiment using 20 yak calves that were divided equally into two groups based on feeding milk substituter; the control group was fed with alfalfa hay; while the treatment group was provided with alfalfa hay and starter; and the treatment group was fed with alfalfa hay and starter. The RAS group has significantly improved the growth of yak calves during the pre-weaning period, including increases in body weight, chest girth, and ruminal formation. metabolites were found between the RA group and the RAS group, of which 15 metabolites were upregulated and 22 metabolites were downregulated in comparison to the RA group, while 22 metabolites were downregulated. ".

Source link: https://doi.org/10.3389/fmicb.2022.821613


Comprehensive Analysis of the Relationships Between the Gut Microbiota and Fecal Metabolome in Individuals With Primary Sjogren’s Syndrome by 16S rRNA Sequencing and LC–MS-Based Metabolomics

"The gut microbiota has been attributed to primary Sjogren'u2019s syndrome," says the author, but the medical implications of these conditions are often vague. " We investigated the fecal microbiota in 30 patients with ps and 20 healthy controls through 16S rRNA gene amplification and sequencing; at the same time, the fecal metabolome was defined by ultrahigh-performance liquid chromatography; U2013mass spectrometry. We discovered that the bacterial composition of pSS patients was substantially different from that of HCs. Increased abundances of proinflammatory microbes, especially Escherichia-Shigella, have been identified in the pampered gut microbiota, as well as decreased numbers of anti-inflammatory microbes. The correlation analysis showed that there were some correlations between the microbiota and metabolism in pSS patients. In addition, an abundance of Escherichia-Shigella was found to be correlated with elevated levels of four metabolites. The gut microbiota in pSS patients is characterized by a specific combination of proinflammatory changes and metabolic states, according to our findings.

Source link: https://doi.org/10.3389/fimmu.2022.874021

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions