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16S rRNA Sequencing - DOAJ

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Last Updated: 10 November 2022

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Integrative Analysis of the Nasal Microbiota and Serum Metabolites in Bovines with Respiratory Disease by 16S rRNA Sequencing and Gas Chromatography/Mass Selective Detector-Based Metabolomics

BRD from various farms in Yinchuan, Ningxia, China, were analyzed by 16S RNA sequencing and metagenomic analysis in our research. We found a significant difference in nasal microbial diversity among diseased and healthy bovines, particularly the relative abundance of Mycoplasma bovis and Pasteurella increased. Moreover, serum metabolomic analysis showed increased amounts of metabolites in BRD, and that a drastic decrease in lactic acid and sarcosine could impair bovines' ability to produce electricity and an immune response to pathogenic bacteria. lactic acid was positively related to Gammaproteobacteria and Bacilli, while negatively correlated with Mollicutes, based on the correlation analysis of microbial diversity and the metabolome. BRD microbial populations and serum metabolites were characterized by integrative analysis in summary.

Source link: https://doi.org/10.3390/ijms231912028


Insight into the Fecal Microbiota Signature Associated with Growth Specificity in Korean Jindo Dogs Using 16S rRNA Sequencing

Previous studies have found that juvenile and adult dogs' microbiomes have significantly different compositions and characteristics. In this research, we aimed to advance our knowledge by determining the difference in fecal microbiome between young and adult dogs by analyzing the feces of 4-month and 16-month-old Jindo dogs, a domestic Korean breed. The 16-month-old group had a high incidence of Bacteroides, according to the comparison results, although the 4-month-old group had a relatively high prevalence of the Lactobacillus genus. The relative fatty metabolism of 4-month-old dogs in 4-month-old dogs was confirmed by microbial function prediction studies. Utilizing microbial function prediction results, glucose metabolism was determined in 16-month-old dogs. Overall, we found genetic and functional differences between genes of the gut microbial population in juveniles and adults. These microbial community profiles can be used as references for future studies into the microbiome associated with health and growth in the canine population.

Source link: https://doi.org/10.3390/ani12192499

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions