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16S rRNA Sequencing - Crossref

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Last Updated: 10 November 2022

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Microbiota Succession of Whole and Filleted European Sea Bass (Dicentrarchus labrax) during Storage under Aerobic and MAP Conditions via 16S rRNA Gene High-Throughput Sequencing Approach

The 16S rRNA High-Throughput Sequencing method was used to investigate the characteristics of bacterial communities of whole and filledeted European sea bass under several storage temperatures under aerobic and Modified Atmosphere Packaging conditions in the present work. Pseudomonas appeared alongside Acinetobacter in both whole and frozen fish, at a similar temperature, in addition to Acutetobacter aerobic storage, and the presence of both Erwinia and Serratia in whole fish was impressive. Overall, the current research provides valuable insight into the storage fate and spoilage status of whole and filleted European sea bass, indicating that various handling and storage conditions influence sea bass's shelf life, increasing or delaying the dominance of Specific Spoilage Organisms, which is related to a large extent to the formation of their consortium that is responsible for product degradation in tandem.

Source link: https://doi.org/10.3390/microorganisms10091870


Diversity and functional profile of gut symbiotic bacteria between Lysinibacillus sphaericus C3-41 susceptible and resistant Culex quinquefasciatus Say as revealed by 16S rRNA gene high-throughput sequencing

In Culex pipiens quinquefasciatus, the relationship between the symbiotic gut bacteria and insect resistance is unclear, according to previous studies, but the connection between the symbiotic gut bacteria and insect resistance to Lysinibacillus sphaericus C 3 -41 is uncertain, but what happens between the symbiotic gut bacteria is unknown. High-throughput 16S RNA sequencing of quinquefasciatus that were resistant or susceptible to L. sphaericus C3 -41 was investigated by high-throughput 16S RNA sequencing. The NMDS and unweighted unifrac PCoA results also showed significant differences between the resistant and vulnerable strains' symbiotic gut bacterial populations, implying that bacterial pesticides may alter bacterial composition.

Source link: https://doi.org/10.3389/fmicb.2022.991105


Analysis of the Composition and Functions of the Microbiome in Diabetic Foot Osteomyelitis Based on 16S rRNA and Metagenome Sequencing Technology

Using 16S rRNA sequencing and metagenome sequencing methods, this prospective observational study examined the microbiome and its function in diabetic foot osteomyelitis and posttraumatic foot osteomyelitis in patients with diabetic foot osteomyelitis and posttraumatic foot osteomyelitis. Spearman's study was used to investigate the relationship between dominant species and clinical characteristics of patients with DFO. The DFO group had a much larger microbial population than in the PFO group, according to the PFO. Prevotella denticola, Prevotella jejuni, and Prevotella fusca had a positive correlation with the duration of diabetic foot disease in the past. The microbial functional genes were more prevalent in the DFO group than in the PFO group.

Source link: https://doi.org/10.2337/db20-0503


Comparison of iSeq and MiSeq as the two platforms for 16S rRNA sequencing in the study of the gut of rat microbiome

Abstract Amplicon-based next-generation sequencing of the 16S ribosomal RNA regions of the 16S is a culture-free method used to identify and analyze Procaryota populations within a given population. The prokaryotic 16S RNA gene encodes conserved regions and nine distinct regions that are commonly used for phylogenetic classification of genus or species in a variety of microbial populations. In 20 fecal rat samples, the most significant similarities and differences of 16S microbiome sequencing were identified. According to 16S Metagenomic Sequencing Library Preparation for the 16S' V3 and V4 regions, genetic libraries were developed according to 16S Metagenomic Sequencing Library Preparation. Using iSeq technology, the species richness obtained using iSeq technology was lower than MiSeq. Microbiota diversity ranged from the class level to the species level in samples sequences on two different research platforms, according to the beta diversity estimation. Compared to the MiSeq, the iSeq's u2022 iSeq platform has the ability to shorten the sequencing time three times.

Source link: https://doi.org/10.1007/s00253-022-12251-z


16S rRNA gene sequencing of stool samples collected from patients with latent tuberculosis infection before, during, and two months after treatment with 3HP or 4R

Abstract aims : We present 16s rRNA gene sequencing and sample results from a pilot observational cohort study to determine the intestinal microbiota alterations in patients with latent tuberculosis infection treated with a three- to four-month course on a rifamycin-based regimen. Following exposure to the rifamycins and document recovery to baseline two months after treatment ended, we wanted to document changes to the gut microbiota. In addition, phosphate buffer washes of the LTBI participants' stool samples were analyzed by a validated LC-MS assay to determine concentrations of the parent and partially active metabolite rifamycins.

Source link: https://doi.org/10.21203/rs.3.rs-2199576/v1


Exploring the Association between Gut and Urine Microbiota and Prostatic Disease including Benign Prostatic Hyperplasia and Prostate Cancer Using 16S rRNA Sequencing

We investigated stool and voided urine samples from patients with benign prostatic hyperplasia or prostate cancer, as well as a control group to see the potential connection between human microbiota and prostate cancer, as well as a control group, with the aim of finding correlations and pathogenic taxonomic units. Alcaligenes, Pseudomonas, Lactobacillus, Akkermansia, and Cetobacterium were among the top five microbial genera with significant differences between the BPH and control groups, according to Alcaligenes, Pseudomonas, Lactobacillus, Akkermansia, and Cetobacterium, the top five microbial genera that displayed significant differences between the top five microbial genera that displayed significant differences between the top five ma, Lactobacillus, Akkermans, Cetobacterium, the top five ma We found that the urine microbiota composition of the BPH and PC groups was different from that of the control group's.

Source link: https://doi.org/10.3390/biomedicines10112676


Investigation of bacterial diversity using 16S rRNA sequencing and prediction of its functionalities in Moroccan phosphate mine ecosystem

Abstract Native plants in arid environments may have some specific microbial communities that may help them maintain their growth and resistance to harsh environments. Using six hypervariable regions of the 16S rRNA, the goal of this research was to investigate the bacterial communities in some native plants and samples of the Moroccan phosphate mine ecosystem by determining the percentages of taxonomic identification using six hypervariable regions of the 16S rRNA. Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes are among the three wild plants in Morocco's Moroccan phosphate mine's remarkable bacterial diversity, including Prothrobacteria, Proteobacteria, Bacteroidetes, and Firmicutes.

Source link: https://doi.org/10.1038/s41598-022-07765-5


16S rRNA sequencing-based evaluation of the protective effects of Hua-Zhuo-Jie-Du on rats with chronic atrophic gastritis

Abstract Background: The intestinal flora disruption is a pathogenic agent for persistent atrophic gastritis. Hua-Zhuo-Du has been shown to be a safe Chinese herbal treatment for treating CAG. However, the effects of HZJD on CAG's intestinal flora are uncertain. Using 16S rRNA gene sequencing, we investigated the controlling effects of HZJD on intestinal microbes in CAG rats. Methods For quality control of HZJD preparations, a high-performance liquid chromatography analysis was used. Based on a KEGG function prediction, differential function analysis was used in PCA. HZJD significantly improved the pathological stability of the gastric mucosa in CAG rats, according to H&E staining. CAG rats' blood pressures were significantly higher in the Chao1 and ACE indices in comparison to the N group. Conclusion HZJD had a therapeutic effect on CAG rats' intestinal flora.

Source link: https://doi.org/10.1186/s12906-022-03542-z


Molecular Identification of Commercial Fish Maws by DNA Sequencing of 16S rRNA and Cytochrome c Oxidase I Genes

ABSTRACT Fish maws have long been used as medicinal tonics and as a valuable food source in Southeast Asia. However, due to a lack of taxonomic characteristics, it is difficult to determine the original species of fish maws sold in supermarkets. In the present study, 37 types of commercial fish maws from various medical product markets were tested, and gene sequences were successfully obtained from ca. The identification results allowed us to learn more about the fish species that were commercial on the fish maw market, as well as an indicator of potential control of endangered or endangered fish species. A probable correlation between the molecular characteristics and morphological characteristics of fish maws was also established, providing both buyers and retailers with an important clue in determining the origins of fish maws.

Source link: https://doi.org/10.4315/jfp-22-121


Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencing

Microbial oxidation of methane contributes to Arctic carbon cycling, potentially reducing methane emissions from lakes, but little is known about methane cycling in Arctic lakes' water column. We determined carbon and hydrogen isotopes and microbial community composition down the water column of Teardrop lake, under open-water conditions using stable isotope and 16S rRNA gene sequencing. Based on geochemical and microbial findings, the methane cycling in Teardrop Lake is decoupled from a more traditional depth dependent model in which the predominant source of methane is found in the anoxic sediments. To constrain the sensitivity of the methane cycle to future climate change, it is therefore important to determine linkages between depth-dependent microbial dynamics and methane biogeochemistry.

Source link: https://doi.org/10.3389/fenvs.2022.884133

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions