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16S rRNA - Springer Nature

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Last Updated: 10 June 2022

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Integrating the salivary microbiome in the forensic toolkit by 16S rRNA gene: potential application in body fluid identification and biogeographic inference

"According to forensic scientists, forensic researchers have been attempting to find new specific molecular markers to refine the existing saliva identification technique. " In this research, 16S RNA sequencing technology was used to sequence the V3-V4 hypervariable regions in saliva samples from five countries in order to highlight the role of geographic location in saliva test heterogeneity. In addition, the microbial community in the saliva samples in this study was significantly different from that in previous studies' vaginal secrets, semen, and skin samples. Moreover, we developed a prediction model based on the random forest algorithm that could distinguish saliva from various regions at different locations at the genus level, although the model has a certain likelihood of misjudgment that needs more detailed study. Overall, the microbial community data in saliva stains may have a place in body fluid identification and biogeographic inference. ".

Source link: https://doi.org/10.1007/s00414-022-02831-z


Association of Candidatus Phytoplasma asteris (16SrI-B subgroup) with Adenium obseum phyllody revealed by 16S rRNA gene sequence

During a survey of flower nursery at Rajmundary, Andhra Pradesh in 2016, "Adenium plants with leaf malformations like small narrow leaves differed from their normal pattern, leaf yellowing, and misformation in flowering branches to leafy rosettes. " Candidatus Phytoplasma asteris, as measured by sequence analysis using BLASTn, similarity coefficient, and virtual RFLP of 16S rRNA sequences demarcated the species of phytoplasma associated with desert rose as Candidatus Phytoplasma asteris. A desert rose isolate from the study was found to be grouped with Ca strains in a 16S rRNA sequence clustered with strains of Ca. In A. obesum in India, a P. asteris strain with leaf and flower malformations is present in a leaf and flower malformation.

Source link: https://doi.org/10.1007/s42360-022-00474-z


16S rRNA Gene Sequence Identification of Cultivable-Bacterioplankton Between Ambient Water and Gastrointestinal Tract (GIT) of Resident Teleost

"The bacterial colonies in O's ambient water and the gastrointestinal tract have been documented. " Both genera were predominant, but Actinobacteria and Bacteroidetes were more prevalent in the ambient water relative to the GIT, indicating Proseobacteria and Bacilli as the highest diversity. Nevertheless, the relative abundance of u03b3-proteobacteria and Bacilli on both classes excelled, with Bacillus and Pseudomonas being the most abundant operational taxonomic units.

Source link: https://doi.org/10.1007/s12088-021-00992-w


Description of Culex (Culex) kodaikanalensis sp. nov. (Diptera: Culicidae) from India with phyletic analysis using mitochondrial cytochrome oxidase I and 16S rRNA genes

"Culex mosquitoes are important vectors of many pathogens to both humans and animals. " The present research reveals the true species of science as Culex kodaikanalensis Suman, Veer, and Chandra sp. In addition, the length of pale spots on R_4+5 and Anal veins of Cx is also measured. The kodaikanalensis can even differ from other subgroups. In comparison to other culicine species being the nearest to Culex gaugleri, kodaikanalensis developed a strong similarity with Mimeticus subgroup species. At high altitudes of Kodaikanal hills in Tamil Nadu, India, Culex kodaikanalensis is common.

Source link: https://doi.org/10.1007/s42690-022-00825-3


Wolbachia 16S rRNA haplotypes detected in wild Anopheles stephensi in eastern Ethiopia

Both P. falciparum and P. vivax were found in eastern and central Ethiopia, with "Anopheles stephensi, an invasive vector normally found in South Asia and the Middle East" being distributed. The discovery of this vector in the Horn of Africa, as well as widespread insecticide resistance, requires that new vector control strategies be investigated in order to prevent malaria transmission in the region. Wolbachia could be used to monitor the mosquito population through suppression or even reduce malaria transmission by population replacement. Stephensi samples were obtained from eastern Ethiopia in 2018 and tested for Wolbachia using a 16S targeted PCR assay and multilocus strain typing PCR assays. The Ethiopian haplotype 2 is distinguished from other sequences in the superclade by a bootstrap value of 89 kilometers. In wild An", these results were the first signs of natural Wolbachia populations.

Source link: https://doi.org/10.1186/s13071-022-05293-9

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions