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16S rRNA - Europe PMC

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Last Updated: 10 June 2022

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The Alteration of Intrauterine Microbiota in Chronic Endometritis Patients Based on 16s rRNA sequencing Analysis

"Among them, patients with persistent endometritis were enrolled in the CE group and others in the non-CE group. " Conclusions The alpha level in the CE group was significantly higher than that in the non-CE group. Actinobacteriota and Cyanobacteria in the CE group were significantly higher than in the non-CE group. Lactobacillus dominated in all samples, although they had lower numbers in CE group than in the non-CE group at a genus level. The morphology and distribution of intrauterine microbiota in the CE group were different from that non-CE group, with lower abundance of Lactobacillus and a significantly higher number of Pseudomonas and Cutibacterium in comparison to non-CE groups. This report detailed the change in intrauterine microbiota in the CE patients', which could raise the diagnosis rate of CE and aid in the selection of antibiotic therapy for CE patients. ".

Source link: https://europepmc.org/article/PPR/PPR501479


Longitudinal 16S rRNA Sequencing Reveals Relationships among Alterations of Gut Microbiota and Nonalcoholic Fatty Liver Disease Progression in Mice.

"Nonalcoholic fatty liver disease is a prevalent and progressive disease spectrum, ranging from nonalcoholic fatty liver to nonalcoholic steatohepatitis, but there is no effective treatment or noninvasive diagnostic system for NASH. " Using 16S RNA sequencing, the present study examined longitudinal variation of gut microbiota in the Western diet-induced murine NAFLD model. Long-term WD intervention changes microbial richness and composition in the intestine, influencing characteristic microbial appearance similarity to each NAFLD stage. These results show that a core group of gut microbiome, specifically BA-related taxa, may be used as a noninvasive diagnostic tool for NAFLD and NASH. These findings may help determine the role of gut microbiota in NAFLD progression and the development of novel noninvasive diagnostic tools for NAFLD based on microbial biomarkers.

Source link: https://europepmc.org/article/MED/35647690


Wolbachia 16S rRNA haplotypes detected in wild Anopheles stephensi in eastern Ethiopia.

"Anopheles stephensi," an invasive vector that is typical in South Asia and the Middle East, has been found in eastern and central Ethiopia and is capable of transmitting both P. falciparum and P. vivax. In order to prevent malaria transmission, the discovery of this vector in the Horn of Africa alongside widespread insecticide resistance means that new vector control strategies must be investigated. Wolbachia could be used to monitor the mosquito population by eradication or in some cases, malaria transmission could be reduced by population transfer. However, Wolbachia is present in wild An. The Ethiopian haplotype 2 is distinguished from other sequences in the superclade by a bootstrap value of 89. These results include the first evidence of natural Wolbachia populations in wild An's wild An.

Source link: https://europepmc.org/article/MED/35610655


Combined 1 H NMR fecal metabolomics and 16S rRNA gene sequencing to reveal the protective effects of Gushudan on kidney-yang-deficiency-syndrome rats via gut-kidney axis.

"Gushudan, a common Chinese medicine prescription with the benefits of bone strengthening and nourishing kidney, and it has been used to treat osteoporosis for years. Many anti-osteoporosis effects of GSD have been investigated in previous studies based on metabolomics. However, the precise mechanism of GSD on kidney tonifying and its changes in gut microbiota are still unclear. The gut-kidney axis and 1 H NMR metabolomics protocols were combined in this research to comprehensively investigate the microbiota and metabolic changes in kidney-yang-deficiency-syndrome rats and 16 S NRNA gene sequencing techniques to determine GSD's protective mechanism. According to the latter, 12 out of 31 suspected metabolites and the presence of 11 out of 16 potential microbial biomarkers related to KYDS had regulated dramatically, respectively. "Understandably, there was a strong correlation between altered fecal metabolites and a spora of genus microflora.

Source link: https://europepmc.org/article/MED/35623116


A 16S rRNA Gene-Based Metabarcoding of Phosphate-Rich Deposits in Muierilor Cave, South-Western Carpathians.

"Muierilor Cave" is one of Romania's most significant show caves, with paleontological and archeological traces. Samples were collected from the new chamber and another passage to investigate the relationship between the substrate and the microbial community. Even after hundreds of years of deposition and in areas far from both deposits, a substantial number of these elements could be found in organic deposits inside the cave, although no bat colonies or fossil bones were present in the new chamber. In order to identify caves that can pose bacterial infections in humans, continued monitoring of air and floor microbiology should be considered. The high number of unidentified taxa in a small portion of Muierilor Cave indicates the lack of information about the bacterial diversity in caves that may have potential uses in human health and biochemistry.

Source link: https://europepmc.org/article/MED/35663904


16S rRNA Gene Sequencing Reveals Specific Gut Microbes Common to Medicinal Insects.

"However, there are already few studies comparing insects' medicinal properties with their gut microbes. " In this research, we concentrated on medicinal insects' specific gut microbiota, in the hopes of finding a link between medicinal values and medicinal insect gut microbes. Parabacteroides goldsteinii, Lactobacillus dextrinicus, Bifidobacterium longum subsp. P. goldsteinii and B. infantis, in particular, were probably involved in the anti-inflammatory activity of medicinal insects. Our results showed a correlation between medicinal insects and their gut microbes, providing new growth directions and possibly potential tools for improving medicinal insects' medicinal efficacy.

Source link: https://europepmc.org/article/MED/35651499


Chronic exposure to polyvinyl chloride microplastics induces liver injury and gut microbiota dysbiosis based on the integration of liver transcriptome profiles and full-length 16S rRNA sequencing data.

"However, polyvinyl chloride MPs' effects on the liver and their associated mechanism in mice are uncertain. " According to the presenter, the liver tissue and fecal samples were subjected to RNA sequencing and full-length 16S RNA sequencing analysis. The serum alanine aminotransferase and aspartate aminotransferase levels in the mice exposed to PVC-MPs were significantly higher than those in the control group, indicating hepatic injury, as shown by hispatic histopathological changes. In addition, PVC-MPs' serum and hepatic triglyceride, as well as total bile acid levels were decreased after exposure to PVC-MPs. PVC-MPs exposure decreased the relative abundance of probiotics and increased the number of conditionally pathogenic bacteria in mouse fecal samples, according to the full-length 16S RNA sequencing analysis. In conclusion, continuous PVC-MPs exposure in mice leads to hepatotoxicity and intestinal microbiota dysbiosis, which is a new insight into the dangers that PVC-MPs may pose to human health.

Source link: https://europepmc.org/article/MED/35588832


16S rRNA metagenomics data on the bacterial communities in integrated poultry-fish farm ponds.

"Fish and bird are raised simultaneously in an integrated poultry-fish raising system. " It seems that farmers can now control poultry waste as it is a common practice in IPF farm settings to feed reared fishes with wastes emanating from the poultry. Using the 16S RNA sequencing technique, this paper provides data on the bacterial taxonomic profile and abundance in IPF farm pond water samples. Total DNA was extracted from pond water samples collected by the IPF farm located at Ila-Orangun, Osun State, Southwest N. . . . ia, during two sampling visits using the ZymoBIOMICS 96 MagBead DNA Kit, using ZymoBIOMICS u00ae-96 MagBead DNA Kit. Interestingly, the number of bacterial pathogens commonly associated with human infections was low. The results obtained can help fill a knowledge gap regarding IPF farming's effect on pond bacterial diversity, a key factor for considerations regarding food safety, fish, and public health.

Source link: https://europepmc.org/article/MED/35647232


Evaluation of metagenomic, 16S rRNA gene and ultra-plexed PCR-based sequencing approaches for profiling antimicrobial resistance gene and bacterial taxonomic composition of polymicrobial samples

"Metagenomics outperformed 16S rRNA gene sequencing in accurately reconstructing a mock taxonomic profile and maximizing the identification of diverse wastewater taxa, while 16S rRNA gene sequencing generated more accurate taxonomic results that can be quantitatively incorrect but increase detection of low abundance taxa. " In profiling AMR genes in a mock sample, shotgun metagenomic and AmpliSeq sequencing yielded similar results, but AmpliSeq reported more genes in more detailed, u201creal-world-u201d samples, owing to the sensitivity of detection at the metagenomic sequencing depth used. However, our analysis indicates that both the sequencing and bioinformatics approach used will have a significant effect on outcomes; for AmpliSeq AMR gene profiling, we created AmpliSeek, which outperformed the other pipelines tested and is open source;.

Source link: https://europepmc.org/article/PPR/PPR495379


Comprehensive Analysis of the Relationships Between the Gut Microbiota and Fecal Metabolome in Individuals With Primary Sjogren's Syndrome by 16S rRNA Sequencing and LC-MS-Based Metabolomics.

"The gut microbiota has been traced to primary Sjogren's syndrome," the condition's biological consequences are often ambigu. " We analyzed the fecal microbiota in 30 patients with pSS and 20 healthy controls; At the same time, the fecal metabolism was defined by ultrahigh-performance liquid chromatography-mass spectrometry. The microbiota composition of pSS patients was significantly different from that of HCs, according to our analysis. Increased abundances of proinflammatory microbes, especially Escherichia-Shigella, as well as reduced quantities of anti-inflammatory microbes are present in the pSS gut microbiota. The correlation study showed that there were some correlations between the microbiota and metabolism in pSS patients. The gut microbiota in pSS patients is characterized by a specific combination of proinflammatory changes and metabolic states," according to our findings.

Source link: https://europepmc.org/article/MED/35634334

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions