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16S rRNA - Crossref

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Last Updated: 10 June 2022

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Study on the airway microecology of pulmonary tuberculosis based on 16S rRNA sequencing

"Abstract Background: Pulmonary tuberculosis is a chronic respiratory disease of the respiratory system. " To determine the respiratory microbiota composition of 30 tuberculosis patients and compared to 30 health controls, using high-throughput 16S RNA gene sequencing was used. According to their Gene Xpert results, 30 pulmonary tuberculosis patients were divided into 12 people in the vulnerable group and 18 in the drug-resistant group. The Alpha number of the TB group is lower than that of the H group, and Beta numbers are higher than that of the H group. Fig. 1: Conclusion: Streptococcus in the DS0 group was much higher than that in the DR0 group, leading to respiratory tract flora disorders, in which the relative abundance of Streptococcus was highly different between rifampicin-sensitive and drug-resistant patients. ".

Source link: https://doi.org/10.21203/rs.3.rs-1667910/v1


Molecular Detection of Bartonella Sp. in Wild Ruminants and Analysis of its Genetic Diversity on the Basis of 16S–23S Rrna Intergenic Spacer (ITS)

"Abstract" is a verb that refers to the state of roe deer and red deer infection by Bartonella sp. Bartonella 16S-23S ITS region in isolates of animal tissue was found in North-Western Poland by PCR analysis of ITS and determining the genetic diversity of identified Bartonella species based on molecular analysis of ITS. Bartonella sp. 's DNA Bartonella sp. The collected findings show that roe and red deer are potential reservoirs for Bartonella sp. Sequences of roe and red deer gathered together in a constructed phylogenetic tree, sequences obtained from roe and red deer clumpeted together in built phylogenetic trees.

Source link: https://doi.org/10.2478/v10213-012-0003-7


RNA-Seq and 16S rRNA Analysis Revealed the Effect of Deltamethrin on Channel Catfish in the Early Stage of Acute Exposure

"However, the toxicological reactions of Del on channel catfish remain uncertain. " In the first stage, pathological tests revealed that a high percentage of Del destroyed the intestinale and trunk kidney of channel catfish in the intestine and trunk kidney. After exposure to 0. 5 and 5 bc/L Del, respectively, transcriptome analysis revealed 32 and 1837 differentially expressed genes in channel catfish trunk kidneys. These findings showed that apoptosis is present in channel catfish's trunk kidneys at an early stage of acute exposure to Del. In addition, 16S RNA gene sequencing showed that the intestinal microbiome was significantly different in channel catfish treated with Del. In the early stages of exposure, Del's toxic effects on channel catfish were clearer, and we figured out why fish under Del stress are more vulnerable to microbial infections and slow growth. ".

Source link: https://doi.org/10.3389/fimmu.2022.916100


Inhibition of translation initiation complex formation by GE81112 unravels a 16S rRNA structural switch involved in P-site decoding

The subunit's conformational change in the subunit aids in the production of a tightly packed 30S complex, with mRNA codonu2013tRNA codon U2013anticodon base-pairing. Moreover, in the absence of GE81112 three rRNA helices, h44/h24a, are stable in a disengaged conformation, meaning that their conformation is associated with tRNA/mRNA decoding and transition of the 30S from unlocked to locked state.

Source link: https://doi.org/10.1073/pnas.1521156113


The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3′ end of 16S rRNA

"We reported previously that the protein recognizes nine nucleotides near the end of 16S rRNA's 3rd end" and that this recognition boosts Era's GTP-hydrolyzing activity. " The sequence and helix 45 sequence in all three kingdoms of life are highly conserved. It has been shown that the double mutation greatly affects bacteria's viability. However, whether Era interacts with G1530 and/or h45, and whether such interactions contribute to Erau2019s GTPase activity, were not known. The buildings reveal that Era acknowledges ten nucleotides, including G1530, and that a new generation binds h45. Furthermore, GTPase assay experiments have shown that G1530 does not boost Erau2019s GTPase activity. Although G1530 does not contribute to Era's intrinsic GTPase activity, its interaction with Era is crucial for binding and is essential for the protein to function, leading to the emergence of a new cold-sensitive phenotype of Age.

Source link: https://doi.org/10.1073/pnas.1017679108


Evaluating Starter Feeding on Ruminal Function in Yak Calves: Combined 16S rRNA Sequencing and Metabolomics

"For young ruminants, starter feeding can safely promote rumen formation and growth in rumens, but rumen formation is a significant physiological challenge, since it is not clear for the mechanism of starter feeding stimulating. " In this research, we performed an investigation of ruminal microbiota and their metabolites in yak calves to see how the ruminal microbiota and their metabolites improve the ruminal function. In yak calves, this research used 16S rRNA sequencing with liquid chromatography-mass spectrometry to determine the effects of starter feeding on ruminal microbiota variety and metabolites. The trial was conducted with 20 yak calves that were divided equally into two groups based on feeding milk substituter; the control group was fed with alfalfa hay while the treatment group was fed with alfalfa hay and starter; the treatment group was fed with alfalfa hay and starter. The RAS group notably promoted growth in yak calves during the pre-weaning period, including increases in body mass, chest girth, and rumen formation. A total of 37 metabolites were found between the RAS group and the RAS group, of which 15 metabolites were upregulated and 22 were downregulated, relative to the RA group, and 22 metabolites were downregulated. Upregulated metabolism of the RAS group yak calves was related to carbohydrate metabolism, ubiquinone, and other terpenoid-quinone biosynthesis, according to the metabolic pathway, although the downregulated metabolic pathway was crucial to xenobiotic biodegradation, metabolism, and nucleotide metabolism," according to Metabolic pathway studies.

Source link: https://doi.org/10.3389/fmicb.2022.821613


16S rRNA amplicon sequencing for epidemiological surveys of bacteria in wildlife: the importance of cleaning post-sequencing data before estimating positivity, prevalence and co-infection

"We investigated the possibility for new advancements in 16S RNA amplicon sequencing to enable multiplexing of large numbers of samples required to increase our knowledge of the danger of zoonotic disease transmission among urban rodents in West Africa. " Our approach can help identify pathogenic bacteria in wild populations as in other high-throughput sequencing studies, such as prevalence and patterns of coinfection within individual hosts, in addition to determining essential zoootic risk in studies of zoonotic risk. Bacterial prevalence varies from 0% to 90% of individuals per site, depending on the bacterial taxon, rodent species, and site chosen, with 26% of rodents showing coinfection. The 16S rRNA amplicon sequencing scheme developed here has the advantage over other molecular diagnostic techniques of dealing with a wide range of bacterial pathogens without needing assumptions about their presence in the samples. ".

Source link: https://doi.org/10.1101/039826

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions