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However, the DNA extraction reagents as well as the PCR reagents can contain residual bacterial DNA, which leads to false-positive PCR results. Here, we discuss the findings obtained with a new technique that uses DNA-free reagents for automated DNA extraction and subsequent real time PCR using sterile clinical specimens. These 62 positive clinical specimens were particularly encouraging based on crossingpoint values, and these 62 positive clinical specimens were deemed as medically relevant as well. Culture-positive, but PCR-negative, for S. epidermidis, S. aureus, and C. acnes, the majority of which were culture-positive, but not PCR-negative. For positive specimens, the turnaround times were 4 hours and 1 working day. SYBR Green-PCR's melting-curve analysis aids in the differentiation of specific and unspecific PCR products. Even mixed infections of two bacterial species could be cured by using Ripseq. Conclusions The added benefit of PCR in endocarditis cases is apparent.
Source link: https://europepmc.org/article/MED/35501697
It was previously believed that the microbial community in the esophagus was relatively stable, but it has since been discovered that some esophageal diseases have specific microbial community characteristics. This review expands on existing research on the esophageal squamous cell carcinoma's squamous cell carcinoma's function and interaction of microorganisms in the esophagus in situ, giving it a route for further inquiry into the relationship between esophageal in situ microorganisms and esophageal squamous cell carcinoma. • The microbial community in esophageal cancer tissue and adjacent non-tumor tissues at the phylum level is similar; Streptococcus and Labrys are the most prevalent bacteria in esophageal tumor tissues and nearby non-tumor tissues, respectively; Microbial interactions in tumor tissues and adjacent non-tumor tissues are stronger than those in adjacent non-tumor tissues.
Source link: https://europepmc.org/article/MED/35435458
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