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We investigated the effects of using different sets of 16S rRNA primers on bacterial composition, range, and predicted function in chicken ceca. By GLMs, changes in bacterial relative abundance following to 16S primers were identified. Microbiota were identified by separation of microbiota among four distinct groups of bacterial populations based on an Unweighted UniFrac distance matrix, according to a separate cluster of bacterial populations in four separate clusters. Our results show that selecting particular sets of the 16S rRNA primers can influence microbiota analysis and interpretation of findings in chicken as has been demonstrated before for humans and other animal species.
Source link: https://www.osti.gov/biblio/1816711
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