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14-3-3 Protein - Europe PMC

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Last Updated: 10 June 2022

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Loss of ubiquitin-specific peptidase 18 destabilizes 14-3-3ζ protein and represses lung cancer metastasis.

"Cancer metastasis is the most common cause of cancer-related mortality. " In lung and other cancers, we previously reported an increase in ubiquitin-specific peptide 18 expression. This report investigates whether USP18 deficiency reduced lung cancer metastasis. Small hairpin RNAs and small interfering RNAs were used to achieve a knock-down in lung cancer cells that was not previously achieved by simple hairpin RNAs and small interfering RNAs. Reduced lung cancer growth, wound-healing, migration, and invasion in comparison to controls, as well as markedly reduced murine lung cancer metastases, according to the USP18 knock-down. 14-3-3-3-3 B6 protein was restricted by loss of USP18 in shRNA knock-down lung cancer cells, according to the authors' reports that the 14-3-3-3u03b6 protein was restricted by a lack of USP18. Human lung cancer arrays and syngeneic murine lung cancer metastasis models were reported and extended using 14-3-3-3u03b6 immunohistochemical assays, as well as syngeneic murine lung cancer metastasis models. engineered 14-3-3-3-3. b6 knock-down in lung cancer cell lines and 14-3-3-3-3-3-3ru03b6 rescue experiments that reversed migration and invasion inhibition resulted in preventing lung cancer metastasis. Dr. Howard Johnson's direct involvement in controlling lung cancer metastasis stems from an engineered 14-3-3-3-3-3-3-3b6 knock-down; and 14-3-3-3-3-3-3-3-3-3-u03b6 involvement in controlling lung cancer cell lines and 14-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3 u03b6 initiation inhibition, a, including migration and invasion inhibition.

Source link: https://europepmc.org/article/MED/35387560


The phospho-docking protein 14-3-3 regulates microtubule-associated proteins in oocytes including the chromosomal passenger Borealin.

"We previously found a novel regulatory system in Drosophila oocytes in which the phospho-docking protein 14-3-3 blocks microtubule binding of Kinesin-14/Ncd away from chromosomes. " In the presence or absence of a 14-3-3 inhibitor, proteins from ovary extract were co-sedimented with microtubules. We showed that 14-3-3 binds to Borealin's chaotic region, and this binding is differentiated by two phosphorylations on Borealin. Mutations at these two phospho-sites stifled normal Borealin localization and centromere bi-orientation in oocytes, indicating that phospho-regulation of 14-3-3-3 binding is critical for Borealin localization and function. ".

Source link: https://europepmc.org/article/MED/35666772


Arbuscular Mycorrhizal Fungi Enhanced Drought Resistance of Populus cathayana by Regulating the 14-3-3 Family Protein Genes.

"This is the first review of 14-3-3s in the symbiosis system of forest arbor plants and AMF, and it may help to investigate the effects of 14-3-3s on stress and provide new strategies for improving mycorrhizal seedling cultivation under stress, as well as new strategies for improving mycorrhizal seedling cultivation under stress. " The 14-3-3 protein gene of AMF is not only restricted under drought stress, but also increases AMF's plant drought tolerance by regulating plant signal pathways and drought response genes; however, data on these interactions remains limited and uncertain. Under drought stress, the precise functions of Populus cathayana 14-3-3s are unclear, and the mechanisms of action of these genes in mycorrhizae-induced drought are still unclear. Hence, determining the drought tolerance of the plant's AMF symbiotic plant 14-3-3 gene is of utmost importance in enhancing the plant's drought tolerance.

Source link: https://europepmc.org/article/MED/35612316


Exploring the Binding Mechanism of a Supramolecular Tweezer CLR01 to 14-3-3σ Protein via Well-Tempered Metadynamics.

"A common tactic in chemical biology and drug discovery is using supramolecules for protein function regulation. " Protein function control by selective binding of supramolecules is difficult, due to the presence of multiple binding sites on protein surfaces. However, the tweezer molecule's binding mechanisms to 14-3-3 proteins are still unclear, limiting the production of novel supramolecules targeting the 14-3-3 proteins. According to simulations, K214 could form a robust binding complex with the tweezer; the binding free energy was estimated to be -10. 5 kcal/u00b7mol -1; in particular, simulations demonstrated that K214 could form a strong binding complex with the tweezer; the adhesion barrier height was 3. 7 mol -1. In addition, several other lysine residues on 14-3-3-3. u03c3 were found to be well-recognized by the tweezer, which corresponds with experimental findings, although only K214/tweezer was co-crystallized. ".

Source link: https://europepmc.org/article/MED/35646830

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* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions