12-Hydroxyeicosatetraenoic Acid - DOAJ

Platelets induce free and phospholipid-esterified 12-hydroxyeicosatetraenoic acid generation in colon cancer cells by delivering 12-lipoxygenase

Platelets advertise tumor metastasis by inducing promalignant phenotypes in cancer cells and straight adding to cancer-related thrombotic complications. 12S-hydroxyeicosatetraenoic acid produced by platelet-type 12-lipoxygenase is taken into consideration an essential modulator of cancer transition via unknown mechanisms. Making use of cocultures of human platelets and human colon adenocarcinoma cells and LC-MS/MS, we checked out the effect of platelets on cancer cell biosynthesis of 12S-HETE and its esterification into PLs and whether platelet capability to transfer its molecular freight could play a role. In cancer cells exposed to platelets, endogenous yet not exogenous 12S-HETE added to modifications in EMT gene expression, minimized by three structurally unconnected 12-LOX preventions. Finally, we revealed that platelets cause the generation of primarily esterified 12-HETE in colon cancer cells following mEV-mediated delivery of 12-LOX. The alteration of cancer cells cell phospholipids by 12-HETE may functionally influence cancer cells cell biology and stand for a novel target for anticancer agent growth.

Source link: https://doi.org/10.1016/j.jlr.2021.100109

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